Name: Uric Acid-L (R1-R2)
Brand: Reckon Diagnostics
SKU: CC3-URK.20M

Photographed: the 2 x 20 ml pack, stock code CC3-URK.20M.

R1-R2 format. 2 pack sizes, 40 to 120 ml.

Choose a pack size

2 x 20 mlCC3-URK.20M 6 x 20 mlCC3-URK.20MU

Stock code CC3-URK.20M

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Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

Uric Acid-L (R1-R2) is a liquid reagent kit for the quantitative estimation of uric acid in serum or plasma, by the uricase method after Fossati et al. Uricase converts uric acid to allantoin and hydrogen peroxide, which peroxidase uses with 4-aminoantipyrine and TOOS to form a coloured complex read at 546 nm. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Enzymatic uricase-peroxidase
Sample: Serum
Sample volume: 50 µl (0.05 ml)
Wavelength: 546 nm (530 to 570 nm, or a yellow filter)
Linearity: Up to 20 mg/dl
Storage: 2 to 8 °C

Full specification20 items

Category: Clinical chemistry
Format: R1-R2
Pack: 40 to 120 ml
Pack sizes: 2 to choose from
Datasheet: Published
Method: Enzymatic uricase-peroxidase, end point
Sample: Serum: fresh, clear, fasting, no hemolysis; heparin plasma may be used
Sample volume: 50 µl (0.05 ml)
Reagent volume: 1.0 ml of working reagent
Wavelength: 546 nm (530 to 570 nm, or a yellow filter)
Temperature: 37 °C
Incubation: 10 minutes at 37 °C
Standard: 6 mg/dl, included
Reagents: R1 and R2, liquid; working reagent is 4 parts R1 to 1 part R2
Working reagent: 4 weeks at 2 to 8 °C
Colour stability: 1 hour, protected from direct light
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC3-URK.20M, CC3-URK.20MU
Made in: Vadodara, Gujarat, India

Performance2 figures

Linearity: Up to 20 mg/dl; dilute higher samples with 0.9% saline
Interference: None from hemoglobin up to 500 mg/dl, free bilirubin up to 15 mg/dl, lipemia (as triglycerides) up to 1000 mg/dl

Procedure at a glance5 steps

Pour 1 bottle of R2 into the R1 bottle and wait 2 minutes, or mix 4 parts of R1 with 1 part of R2 as needed.
Pipette 1.0 ml of working reagent into the blank, standard and test tubes; add 0.05 ml of standard and of sample to their tubes.
Mix and incubate at 37 °C for 10 minutes.
Read the test and the standard against the reagent blank at 546 nm.
Uric acid (mg/dl) = absorbance of test ÷ absorbance of standard × 6; multiply by 59.5 for µmol/L.