Name: Adenosine Deaminase (ADA)
Brand: Reckon Diagnostics
SKU: CC3-ADA.025

Reagent bottle: no photograph of this pack is published yet

Drawing, not a photograph. This pack has not been photographed yet; the stock codes and pack sizes below are the specification.

2 pack sizes, 20 to 40 ml.

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1 x 20 mlCC3-ADA.025 2 x 20 mlCC3-ADA.25U

Stock code CC3-ADA.025

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Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

Adenosine Deaminase (ADA) reagent is an enzymatic assay for the in vitro quantitative detection of adenosine deaminase in human serum on semi- and fully automated clinical chemistry analyzers. ADA converts adenosine to inosine, and a coupled PNP, xanthine oxidase and peroxidase reaction forms a quinoneimine dye that is monitored kinetically. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Enzymatic, kinetic (increase)
Sample: Non-hemolytic serum
Sample volume: 10 µl semi-automated
Wavelength: 546 nm (700 nm secondary, optional)
Linearity: 2 to 200 U/L (R² ≥ 0.990)
Storage: 2 to 8 °C

Full specification15 items

Category: Clinical chemistry
Pack: 20 to 40 ml
Pack sizes: 2 to choose from
Datasheet: Published
Method: Enzymatic, kinetic (increase)
Sample: Non-hemolytic serum
Sample volume: 10 µl semi-automated; 6 µl fully automated
Reagents: R1 and R2, liquid, ready to use; lyophilised calibrator included
Wavelength: 546 nm (700 nm secondary, optional)
Temperature: 37 °C
Incubation: 5 minutes, then 3 minutes after R2; read for 2 minutes
On-board stability: 30 days
Storage: 2 to 8 °C; do not freeze. Reconstituted calibrator: 3 days at 2 to 8 °C
Stock codes: CC3-ADA.025, CC3-ADA.25U
Made in: Vadodara, Gujarat, India

Performance3 figures

Linearity: 2 to 200 U/L (R² ≥ 0.990)
Precision: CV ≤ 5%
Interference: None up to bilirubin 60 mg/dl, hemoglobin 50 mg/dl, intralipid 750 mg/dl

Procedure at a glance4 steps

Mix 10 µl of sample with 375 µl of R1 and incubate for 5 minutes at 37 °C.
Add 125 µl of R2, mix, and incubate for 3 minutes at 37 °C.
Read the absorbance at 546 nm continuously for 2 minutes and calculate ΔA/min.
ADA (U/L) = ΔA/min × factor, with the factor worked out as the datasheet sets out.