Photographed: the 2 x 10 ml pack, stock code CC3-AMY.03M.
Single liquid format. 2 pack sizes, 20 to 50 ml.
Stock code CC3-AMY.03M
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
Intended use: α-Amylase-L (Single Liquid) is a ready-to-use reagent for the quantitative estimation of α-amylase in serum or plasma, by a kinetic method using the blocked chromogenic substrate GAL-G2-CNP. α-Amylase releases 2-chloro-4-nitrophenol (CNP), measured at 405 nm in proportion to its activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.
- Method
- Kinetic, GAL-G2-CNP substrate
- Sample
- Serum or heparinised plasma
- Sample volume
- 50 µl (0.050 ml)
- Wavelength
- 405 nm
- Linearity
- Up to 1500 IU/L
- Storage
- 2 to 8 °C
Full specification20 items
- Category
- Clinical chemistry
- Format
- SINGLE LIQUID
- Pack
- 20 to 50 ml
- Pack sizes
- 2 to choose from
- Datasheet
- Published
- Method
- Kinetic, GAL-G2-CNP substrate
- Sample
- Serum or heparinised plasma; not EDTA, oxalate or citrate, which inhibit amylase
- Sample volume
- 50 µl (0.050 ml)
- Reagent volume
- 1.0 ml
- Wavelength
- 405 nm
- Temperature
- 37 °C
- Readings
- 4, at 30-second intervals after a 60-second delay
- Factor
- 1628
- Reagents
- Single liquid reagent, ready to use
- Opened vial
- Stable for 4 weeks at 2 to 8 °C
- Contamination
- Keep saliva and cough away from the reagent: both contain amylase
- Caution
- Contains sodium azide; do not ingest
- Storage
- 2 to 8 °C, until the expiry date on the label
- Stock codes
- CC3-AMY.03M, CC3-AMY.3MU
- Made in
- Vadodara, Gujarat, India
Performance1 figure
- Linearity
- Up to 1500 IU/L; dilute higher samples with 0.9% saline
Procedure at a glance4 steps
- Add 0.05 ml of sample to 1.0 ml of reagent and mix immediately.
- Read the absorbance at 405 nm at exactly 60 seconds, then 3 more times at 30-second intervals, at 37 °C.
- Work out the mean change in absorbance per minute (ΔA/min).
- Amylase (IU/L) = ΔA/min × 1628.
Every stock code.
α-Amylase-L (Single Liquid) ships in 2 pack sizes, each with its own stock code.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| CC3-AMY.03M | 2 x 10 ml |  |
Datasheet PDF, CC3-AMY.03M |
| CC3-AMY.3MU | 2 x 25 ml |  |
Datasheet PDF, CC3-AMY.3MU |
Questions and answers.
α-Amylase-L (Single Liquid): what sample does it need?
The datasheet gives the sample as Serum or heparinised plasma; not EDTA, oxalate or citrate, which inhibit amylase. The sample volume is 50 µl (0.050 ml).
α-Amylase-L (Single Liquid): what method does it use?
The method on the datasheet is Kinetic, GAL-G2-CNP substrate.
α-Amylase-L (Single Liquid): what wavelength is it read at?
According to the datasheet, it is read at 405 nm.
α-Amylase-L (Single Liquid): what is the linear range?
The datasheet gives the linear range as Up to 1500 IU/L; dilute higher samples with 0.9% saline.
α-Amylase-L (Single Liquid): how should it be stored?
The datasheet gives storage as 2 to 8 °C, until the expiry date on the label.
α-Amylase-L (Single Liquid): what pack sizes are available?
It comes in 2 pack sizes, each with its own stock code: 2 x 10 ml (CC3-AMY.03M), 2 x 25 ml (CC3-AMY.3MU).
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