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CLINICAL CHEMISTRY · Α-AMYLASE-L (SINGLE LIQUID)

α-Amylase-L (Single Liquid)

α-Amylase-L (Single Liquid), 2 x 10 ml

Photographed: the 2 x 10 ml pack, stock code CC3-AMY.03M.

SINGLE LIQUID format. 2 pack sizes, 20 to 50 ml.

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Stock code CC3-AMY.03M

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  • Made and batch-released in Vadodara
  • ISO 13485:2016 quality system
  • Datasheet published: see every stock code below

Product details.

α-Amylase-L (Single Liquid) is a ready-to-use reagent for the quantitative estimation of α-amylase in serum or plasma, by a kinetic method using the blocked chromogenic substrate GAL-G2-CNP. α-Amylase releases 2-chloro-4-nitrophenol (CNP), measured at 405 nm in proportion to its activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method
Kinetic, GAL-G2-CNP substrate
Sample
Serum or heparinised plasma
Sample volume
50 µl (0.050 ml)
Wavelength
405 nm
Linearity
Up to 1500 IU/L
Storage
2 to 8 °C
Full specification20 items
Format
SINGLE LIQUID
Pack
20 to 50 ml
Pack sizes
2 to choose from
Datasheet
Published
Method
Kinetic, GAL-G2-CNP substrate
Sample
Serum or heparinised plasma; not EDTA, oxalate or citrate, which inhibit amylase
Sample volume
50 µl (0.050 ml)
Reagent volume
1.0 ml
Wavelength
405 nm
Temperature
37 °C
Readings
4, at 30-second intervals after a 60-second delay
Factor
1628
Reagents
Single liquid reagent, ready to use
Opened vial
Stable for 4 weeks at 2 to 8 °C
Contamination
Keep saliva and cough away from the reagent: both contain amylase
Caution
Contains sodium azide; do not ingest
Storage
2 to 8 °C, until the expiry date on the label
Stock codes
CC3-AMY.03M, CC3-AMY.3MU
Made in
Vadodara, Gujarat, India
Performance1 figure
Linearity
Up to 1500 IU/L; dilute higher samples with 0.9% saline
Procedure at a glance4 steps
  1. Add 0.05 ml of sample to 1.0 ml of reagent and mix immediately.
  2. Read the absorbance at 405 nm at exactly 60 seconds, then 3 more times at 30-second intervals, at 37 °C.
  3. Work out the mean change in absorbance per minute (ΔA/min).
  4. Amylase (IU/L) = ΔA/min × 1628.

Every stock code.

α-Amylase-L (Single Liquid) ships in 2 pack sizes, each with its own stock code.

Stock codes and pack sizes for α-Amylase-L (Single Liquid)
Stock code Pack Photograph Datasheet
CC3-AMY.03M 2 x 10 ml α-Amylase-L (Single Liquid), 2 x 10 ml Datasheet
CC3-AMY.3MU 2 x 25 ml α-Amylase-L (Single Liquid), 2 x 25 ml Datasheet

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