Photographed: the 2 x 60 ml pack, stock code CC3-CLE.05M.
Single liquid format. 2 pack sizes, 120 to 300 ml.
Stock code CC3-CLE.05M
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
Intended use: Cholesterol-L (Single Liquid) is a ready-to-use liquid reagent for the quantitative estimation of cholesterol in serum or plasma. Cholesterol esterase and cholesterol oxidase release hydrogen peroxide, which with peroxidase, 4-aminoantipyrine and a phenolic compound forms a quinoneimine dye read at 505 nm in proportion to total cholesterol. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.
- Method
- Enzymatic CHOD-PAP, end point
- Sample
- Serum
- Sample volume
- 10 µl
- Wavelength
- 505 nm (505 to 530 nm, or a green filter)
- Linearity
- Up to 1000 mg/dl
- Storage
- 2 to 8 °C
Full specification18 items
- Category
- Clinical chemistry
- Format
- SINGLE LIQUID
- Pack
- 120 to 300 ml
- Pack sizes
- 2 to choose from
- Datasheet
- Published
- Method
- Enzymatic CHOD-PAP, end point
- Sample
- Serum: fresh, clear, fasting, no hemolysis; heparin or EDTA plasma may also be used
- Sample volume
- 10 µl
- Reagent volume
- 1.0 ml
- Wavelength
- 505 nm (505 to 530 nm, or a green filter)
- Flowcell temperature
- 30 °C or 37 °C
- Incubation
- 10 minutes at 37 °C, or 30 minutes at room temperature (25 ± 5 °C)
- Standard
- 200 mg/dl, included
- Reagents
- Single liquid reagent, ready to use; lipid clearing factor included
- Caution
- Contains sodium azide; do not ingest
- Storage
- 2 to 8 °C, until the expiry date on the label
- Stock codes
- CC3-CLE.05M, CC3-CLE.5MU
- Made in
- Vadodara, Gujarat, India
Performance2 figures
- Linearity
- Up to 1000 mg/dl; dilute higher samples with 0.9% saline
- Interference
- None from hemoglobin up to 375 mg/dl, free bilirubin up to 7.5 mg/dl, lipemia (as triglycerides) up to 1000 mg/dl
Procedure at a glance4 steps
- Pipette 1.0 ml of reagent into the blank, standard and test tubes; add 0.01 ml of standard and of sample to their tubes.
- Mix and incubate for 10 minutes at 37 °C, or 30 minutes at room temperature (25 ± 5 °C).
- Read the standard and the test against the reagent blank at 505 nm.
- Cholesterol (mg/dl) = absorbance of test ÷ absorbance of standard × 200.
Every stock code.
Cholesterol-L (Single Liquid) ships in 2 pack sizes, each with its own stock code.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| CC3-CLE.05M | 2 x 60 ml |  |
Datasheet PDF, CC3-CLE.05M |
| CC3-CLE.5MU | 5 x 60 ml |  |
Datasheet PDF, CC3-CLE.5MU |
Questions and answers.
Cholesterol-L (Single Liquid): what sample does it need?
The datasheet gives the sample as Serum: fresh, clear, fasting, no hemolysis; heparin or EDTA plasma may also be used. The sample volume is 10 µl.
Cholesterol-L (Single Liquid): what method does it use?
The method on the datasheet is Enzymatic CHOD-PAP, end point.
Cholesterol-L (Single Liquid): what wavelength is it read at?
According to the datasheet, it is read at 505 nm (505 to 530 nm, or a green filter).
Cholesterol-L (Single Liquid): what is the linear range?
The datasheet gives the linear range as Up to 1000 mg/dl; dilute higher samples with 0.9% saline.
Cholesterol-L (Single Liquid): how should it be stored?
The datasheet gives storage as 2 to 8 °C, until the expiry date on the label.
Cholesterol-L (Single Liquid): what pack sizes are available?
It comes in 2 pack sizes, each with its own stock code: 2 x 60 ml (CC3-CLE.05M), 5 x 60 ml (CC3-CLE.5MU).
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