Name: Creatinine (End Point & Kinetic)
Brand: Reckon Diagnostics
SKU: CC3-CEK.08P

Creatinine (End Point & Kinetic), 2 x 125 ml

Photographed: the 2 x 125 ml pack, stock code CC3-CEK.08P.

Supplied as 250 ml.

Pack size

2 x 125 mlCC3-CEK.08P

Stock code CC3-CEK.08P

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Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

Creatinine (End Point & Kinetic) is a reagent kit for the quantitative estimation of creatinine in serum or urine by a modified Jaffe method, run either kinetically or as a two-step end point. Creatinine reacts with alkaline picrate to form a red complex whose rate of formation, read at 510 nm, is proportional to creatinine concentration. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Modified Jaffe
Sample: Serum
Sample volume: 100 µl (0.1 ml)
Wavelength: 510 nm (505 to 530 nm, or a green filter)
Linearity: Up to 12 mg/dl
Storage: Reagents at room temperature

Full specification17 items

Category: Clinical chemistry
Pack: 250 ml
Datasheet: Published
Method: Modified Jaffe; kinetic or two-step end point
Sample: Serum: fresh, clear, no hemolysis; heparin plasma may be used; urine diluted 1:50 or 1:100
Sample volume: 100 µl (0.1 ml); 0.2 ml in the 2.5 ml procedure
Reagent volume: 1.0 ml of working reagent, or 2.5 ml
Wavelength: 510 nm (505 to 530 nm, or a green filter)
Temperature: 30 °C kinetic; 30 °C or 37 °C end point
Readings: Kinetic: at 20 seconds and 80 seconds
Incubation: End point: 15 minutes at 37 °C, then 5 minutes after the acid reagent
Standard: 2 mg/dl, supplied separately
Reagents: Picrate, alkali and acid reagents; the working reagent is equal volumes of 1 and 2
Caution: Avoid contact with skin, eyes and clothes; use automated pipettes
Storage: Reagents at room temperature; standard at 2 to 8 °C
Stock codes: CC3-CEK.08P
Made in: Vadodara, Gujarat, India

Performance2 figures

Linearity: Up to 12 mg/dl; dilute higher samples with 0.9% saline
Interference: None from hemoglobin up to 50 mg/dl, free bilirubin up to 12 mg/dl, lipemia (as triglycerides) up to 500 mg/dl

Procedure at a glance5 steps

Mix equal volumes of 1 Creatinine and 2 Creatinine as the day's working reagent.
Kinetic: add 0.1 ml of standard or sample to 1.0 ml of working reagent, mix, and read at 510 nm at 20 and 80 seconds.
Kinetic result: creatinine (mg/dl) = (TS2 − TS1) ÷ (ST2 − ST1) × 2.
End point: incubate 1.0 ml of working reagent with 0.1 ml of standard or sample for 15 minutes at 37 °C and read; add 0.05 ml of 3 Creatinine, keep 5 minutes, and read again.
End point result: creatinine (mg/dl) = (TS1 − TS2) ÷ (STD1 − STD2) × 2.