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CLINICAL CHEMISTRY · DRABKIN'S REAGENT (FOR HB)

Drabkin's Reagent (for Hb)

Reagent bottle: no photograph of this pack is published yet

Drawing, not a photograph. This pack has not been photographed yet; the stock codes and pack sizes below are the specification.

2 pack sizes, 1000 to 5000 ml.

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Stock code CC3-DRB.042

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  • Made and batch-released in Vadodara
  • ISO 13485:2016 quality system
  • Datasheet published: see every stock code below

Product details.

Drabkin's Reagent is a reagent kit for the quantitative estimation of hemoglobin in blood by the cyanmethemoglobin method recommended by the International Committee for Standardization in Hematology. Hemoglobin is oxidised to methemoglobin, which reacts with potassium cyanide to form cyanmethemoglobin, measured at 540 nm. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 02-2017. The datasheet governs: read it before use, or ask us.

Method
Cyanmethemoglobin (ICSH recommended)
Sample
Whole blood
Sample volume
0.02 ml
Reading time
After 3 minutes
Wavelength
540 nm (520 to 560 nm, or a green filter)
Linearity
Up to 25 g/dl
Full specification16 items
Pack
1000 to 5000 ml
Pack sizes
2 to choose from
Datasheet
Published
Method
Cyanmethemoglobin (ICSH recommended), end point
Sample
Whole blood; fresh capillary, or venous with anticoagulant
Sample volume
0.02 ml
Reagent volume
5.00 ml
Wavelength
540 nm (520 to 560 nm, or a green filter)
Reading time
After 3 minutes
Standard
Hemoglobin standard 60 mg/dl, supplied separately
Colour stability
24 hours
Caution
Contains potassium cyanide; use automated pipettes and avoid ingestion
Storage
Reagent at room temperature; standard at 2 to 8 °C
Stock codes
CC3-DRB.042, CC3-DRB.42U
Made in
Vadodara, Gujarat, India
Performance1 figure
Linearity
Up to 25 g/dl
Procedure at a glance5 steps
  1. Pipette 5.00 ml of Drabkin's reagent into a test tube.
  2. Add 0.02 ml of blood and mix by inversion.
  3. After 3 minutes, read the absorbance against distilled water at 540 nm (520 to 560 nm, or a green filter).
  4. Read the standard directly, without dilution.
  5. Hb (g/dl) = absorbance of test ÷ absorbance of standard × 15; on a calibrated spectrophotometer, Hb (g/dl) = absorbance of test × 36.77.

Every stock code.

Drabkin's Reagent (for Hb) ships in 2 pack sizes, each with its own stock code.

Stock codes and pack sizes for Drabkin's Reagent (for Hb)
Stock code Pack Photograph Datasheet
CC3-DRB.042 1000 ml Datasheet
CC3-DRB.42U 5 Lts. Datasheet

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