Photographed: the 1000 ml pack, stock code CC3-DRB.042.
Supplied as 1000 ml.
Stock code CC3-DRB.042
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
Intended use: Drabkin's Reagent is a reagent kit for the quantitative estimation of hemoglobin in blood by the cyanmethemoglobin method recommended by the International Committee for Standardization in Hematology. Hemoglobin is oxidised to methemoglobin, which reacts with potassium cyanide to form cyanmethemoglobin, measured at 540 nm. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 02-2017. The datasheet governs: read it before use, or ask us.
- Method
- Cyanmethemoglobin (ICSH recommended)
- Sample
- Whole blood
- Sample volume
- 0.02 ml
- Reading time
- After 3 minutes
- Wavelength
- 540 nm (520 to 560 nm, or a green filter)
- Linearity
- Up to 25 g/dl
Full specification15 items
- Category
- Clinical chemistry
- Pack
- 1000 ml
- Datasheet
- Published
- Method
- Cyanmethemoglobin (ICSH recommended), end point
- Sample
- Whole blood; fresh capillary, or venous with anticoagulant
- Sample volume
- 0.02 ml
- Reagent volume
- 5.00 ml
- Wavelength
- 540 nm (520 to 560 nm, or a green filter)
- Reading time
- After 3 minutes
- Standard
- Hemoglobin standard 60 mg/dl, supplied separately
- Colour stability
- 24 hours
- Caution
- Contains potassium cyanide; use automated pipettes and avoid ingestion
- Storage
- Reagent at room temperature; standard at 2 to 8 °C
- Stock codes
- CC3-DRB.042
- Made in
- Vadodara, Gujarat, India
Performance1 figure
- Linearity
- Up to 25 g/dl
Procedure at a glance5 steps
- Pipette 5.00 ml of Drabkin's reagent into a test tube.
- Add 0.02 ml of blood and mix by inversion.
- After 3 minutes, read the absorbance against distilled water at 540 nm (520 to 560 nm, or a green filter).
- Read the standard directly, without dilution.
- Hb (g/dl) = absorbance of test ÷ absorbance of standard × 15; on a calibrated spectrophotometer, Hb (g/dl) = absorbance of test × 36.77.
Every stock code.
Drabkin's Reagent (for Hb) ships in one pack size.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| CC3-DRB.042 | 1000 ml |  |
Datasheet PDF, CC3-DRB.042 |
Questions and answers.
Drabkin's Reagent (for Hb): what sample does it need?
The datasheet gives the sample as Whole blood; fresh capillary, or venous with anticoagulant. The sample volume is 0.02 ml.
Drabkin's Reagent (for Hb): what method does it use?
The method on the datasheet is Cyanmethemoglobin (ICSH recommended), end point.
Drabkin's Reagent (for Hb): what wavelength is it read at?
According to the datasheet, it is read at 540 nm (520 to 560 nm, or a green filter).
Drabkin's Reagent (for Hb): what is the linear range?
The datasheet gives the linear range as Up to 25 g/dl.
Drabkin's Reagent (for Hb): how should it be stored?
The datasheet gives storage as Reagent at room temperature; standard at 2 to 8 °C.
Drabkin's Reagent (for Hb): what pack sizes are available?
It comes in one pack size: 1000 ml (CC3-DRB.042).
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