Name: G-6-PD (Quantitative)
Brand: Reckon Diagnostics
SKU: CC1-GPD.009

Photographed: the 12 x 1.1 ml pack, stock code CC1-GPD.009.

Supplied as 13.2 ml.

Pack size

12 x 1.1 mlCC1-GPD.009

Stock code CC1-GPD.009

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Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

G-6-PD (Quantitative) is a reagent kit for the quantitative kinetic estimation of glucose-6-phosphate dehydrogenase in erythrocytes. The enzyme, released from lysed red cells, oxidises glucose-6-phosphate while reducing NADP to NADPH, and the rise in absorbance at 340 nm is proportional to G6PD activity; an inhibitor prevents interference from 6-phosphogluconate dehydrogenase. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Kinetic UV, at 340 nm
Sample: Whole blood
Sample volume: 25 µl (0.025 ml) of hemolysate
Wavelength: 340 nm
Storage: 2 to 8 °C
Pack: 13.2 ml

Full specification17 items

Category: Clinical chemistry
Pack: 13.2 ml
Datasheet: Published
Method: Kinetic UV, at 340 nm
Sample: Whole blood: fresh, with citrate, oxalate or heparin; red cells washed and lysed
Sample volume: 25 µl (0.025 ml) of hemolysate
Reagent volume: 1.0 ml of working reagent
Wavelength: 340 nm
Temperature: 30 °C
Readings: 4, at 60-second intervals after a 180-second delay
Also needed: Hemoglobin and red cell count of the whole blood
Reagents: Co-enzyme substrate vials, buffer and lysing reagent; use the lysing reagent cold (0 to 8 °C)
Working reagent: 7 days at 2 to 8 °C
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC1-GPD.009
Made in: Vadodara, Gujarat, India

Procedure at a glance5 steps

Wash 0.1 ml of whole blood 3 times in 2 ml of 0.9% saline; lyse the packed cells in 0.5 ml of cold lysing reagent, keep at 2 to 4 °C for 15 minutes to 2 hours, and centrifuge at 3000 rpm for 5 minutes.
Add 1.1 ml of buffer to a substrate vial, mix, and wait 5 minutes.
Add 0.025 ml of hemolysate to 1.0 ml of working reagent, mix and aspirate.
After 180 seconds, read the absorbance at 340 nm every minute for 3 minutes and work out ΔA/min.
G6PD (U/10¹² RBC) = ΔA/min × 36013 ÷ red cell count (millions/mm³); G6PD (U/g Hb) = ΔA/min × 3601 ÷ Hb (g/dl).