Product details.

GGT is a reagent kit for the quantitative estimation of gamma glutamyl transferase activity in serum or plasma, by the Szasz kinetic method recommended by the Scandinavian Society for Clinical Chemistry and Physiology. GGT converts L-gamma-glutamyl-p-nitroanilide and glycylglycine to p-nitroaniline, and the rate of increase in absorbance at 405 nm is proportional to GGT activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Kinetic, Szasz (GPNA substrate)
Sample: Serum
Sample volume: 100 µl (0.1 ml)
Wavelength: 405 nm
Linearity: Up to 500 IU/L
Storage: 2 to 8 °C

Full specification17 items

Category: Clinical chemistry
Pack: 22 ml
Datasheet: Published
Method: Kinetic, Szasz (GPNA substrate)
Sample: Serum: fresh, clear, no hemolysis; heparin plasma may also be used
Sample volume: 100 µl (0.1 ml)
Reagent volume: 1.0 ml of working reagent
Wavelength: 405 nm
Temperature: 37 °C or 30 °C
Readings: 4, at 30-second intervals after a 60-second delay
Factor: 1158
Reagents: Substrate tablets and buffer; 1 tablet in 1.1 ml of buffer, used after 5 minutes
Working reagent: 14 days at 2 to 8 °C; discard if absorbance reaches 0.8 at 405 nm
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC1-GGT.010
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 500 IU/L; dilute higher samples with 0.9% saline

Procedure at a glance5 steps

Reconstitute 1 GGT tablet with 1.1 ml of buffer, mix gently, and wait 5 minutes.
Add 0.1 ml of sample to 1.0 ml of working reagent and mix immediately.
Read the absorbance at 405 nm at exactly 60 seconds, then 3 more times at 30-second intervals.
Work out the mean change in absorbance per minute (ΔA/min).
GGT (IU/L) = ΔA/min × 1158.