Product details.

CK-NAC is a reagent kit for the quantitative estimation of creatine kinase in serum, by the IFCC method activated with N-acetylcysteine. Creatine kinase forms ATP from creatine phosphate and ADP; hexokinase and G6PDH couple this to NADPH, and the rise in absorbance at 340 nm is proportional to creatine kinase activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2026. The datasheet governs: read it before use, or ask us.

Method: Kinetic, IFCC NAC activated
Sample: Serum
Sample volume: 50 µl (0.05 ml)
Wavelength: 340 nm
Linearity: Up to 1000 IU/L
Storage: 2 to 8 °C

Full specification18 items

Category: Clinical chemistry
Pack: 22 to 100 ml
Pack sizes: 3 to choose from
Datasheet: Published
Method: Kinetic, IFCC NAC activated
Sample: Serum: fresh, clear, fasting, no hemolysis; heparin plasma may also be used
Sample volume: 50 µl (0.05 ml)
Reagent volume: 1.0 ml of working reagent
Wavelength: 340 nm
Temperature: 30 °C or 37 °C
Readings: 4, at 30-second intervals after a 120-second delay
Factor: 3376
Reagents: Enzyme and activator vials dissolved in buffer (1.1 ml, 3 ml or 10 ml); use after 10 minutes
Working reagent: 21 days at 2 to 8 °C
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC1-CKN.006, CC2-CKN.06U, CC2-CKN.06V
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 1000 IU/L; dilute higher samples with 0.9% saline

Procedure at a glance5 steps

Dissolve 1 vial of 1 CK-NAC in its buffer (1.1 ml, 3 ml or 10 ml), mix, and wait 10 minutes.
Add 0.05 ml of sample to 1.00 ml of working reagent and mix immediately.
Read the absorbance at 340 nm at 120 seconds, then 3 more times at 30-second intervals.
Work out the mean change in absorbance per minute (ΔA/min).
CK (IU/L) = ΔA/min × 3376.