Product details.

CK-MB is a reagent kit for the quantitative estimation of creatine kinase-MB activity in serum, by IFCC immunoinhibition. A monoclonal antibody blend fully inhibits CK-M activity, the remaining CK-B activity is measured by the rise in absorbance at 340 nm, and CK-MB activity is twice the CK-B activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2026. The datasheet governs: read it before use, or ask us.

Method: Immunoinhibition, IFCC
Sample: Serum
Sample volume: 50 µl (0.05 ml)
Wavelength: 340 nm
Linearity: Up to 1500 IU/L
Storage: 2 to 8 °C

Full specification19 items

Category: Clinical chemistry
Pack: 22 to 100 ml
Pack sizes: 3 to choose from
Datasheet: Published
Method: Immunoinhibition, IFCC; kinetic
Sample: Serum: fresh, clear; heparin plasma may also be used; CK-MB stable 6 to 8 hours at 2 to 8 °C
Sample volume: 50 µl (0.05 ml)
Reagent volume: 1.0 ml of working reagent
Wavelength: 340 nm
Temperature: 37 °C
Readings: From 300 seconds, at 30-second intervals
Factor: 6752
Limitation: Assumes CK-BB is negligible; macro CK-BB can give a falsely high CK-MB
Reagents: Enzyme-activator vials dissolved in the buffer-antibody (1.1 ml, 3 ml or 10 ml); use after 15 minutes
Working reagent: Stable for 21 days after reconstitution
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC1-CKM.007, CC2-CKM.07U, CC2-CKM.07V
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 1500 IU/L; dilute higher samples with 0.9% saline

Procedure at a glance5 steps

Dissolve 1 bottle of 2 CK-MB in the 1 CK-MB buffer (1.1 ml, 3 ml or 10 ml) and wait 15 minutes.
Warm 1.0 ml of working reagent at 37 °C for 5 minutes, then add 0.05 ml of sample.
Mix and incubate at 37 °C for 5 minutes.
Read the absorbance at 340 nm from 300 seconds at 30-second intervals, and work out ΔA/min.
CK-MB (IU/L) = ΔA/min × 6752.