Photographed: the 20 x 1.1 ml pack, stock code CC1-CKM.007.
3 pack sizes, 22 to 100 ml.
Stock code CC1-CKM.007
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
Intended use: CK-MB is a reagent kit for the quantitative estimation of creatine kinase-MB activity in serum, by IFCC immunoinhibition. A monoclonal antibody blend fully inhibits CK-M activity, the remaining CK-B activity is measured by the rise in absorbance at 340 nm, and CK-MB activity is twice the CK-B activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 04-2026. The datasheet governs: read it before use, or ask us.
- Method
- Immunoinhibition, IFCC
- Sample
- Serum
- Sample volume
- 50 µl (0.05 ml)
- Wavelength
- 340 nm
- Linearity
- Up to 1500 IU/L
- Storage
- 2 to 8 °C
Full specification19 items
- Category
- Clinical chemistry
- Pack
- 22 to 100 ml
- Pack sizes
- 3 to choose from
- Datasheet
- Published
- Method
- Immunoinhibition, IFCC; kinetic
- Sample
- Serum: fresh, clear; heparin plasma may also be used; CK-MB stable 6 to 8 hours at 2 to 8 °C
- Sample volume
- 50 µl (0.05 ml)
- Reagent volume
- 1.0 ml of working reagent
- Wavelength
- 340 nm
- Temperature
- 37 °C
- Readings
- From 300 seconds, at 30-second intervals
- Factor
- 6752
- Limitation
- Assumes CK-BB is negligible; macro CK-BB can give a falsely high CK-MB
- Reagents
- Enzyme-activator vials dissolved in the buffer-antibody (1.1 ml, 3 ml or 10 ml); use after 15 minutes
- Working reagent
- Stable for 21 days after reconstitution
- Caution
- Contains sodium azide; do not ingest
- Storage
- 2 to 8 °C, until the expiry date on the label
- Stock codes
- CC1-CKM.007, CC2-CKM.07U, CC2-CKM.07V
- Made in
- Vadodara, Gujarat, India
Performance1 figure
- Linearity
- Up to 1500 IU/L; dilute higher samples with 0.9% saline
Procedure at a glance5 steps
- Dissolve 1 bottle of 2 CK-MB in the 1 CK-MB buffer (1.1 ml, 3 ml or 10 ml) and wait 15 minutes.
- Warm 1.0 ml of working reagent at 37 °C for 5 minutes, then add 0.05 ml of sample.
- Mix and incubate at 37 °C for 5 minutes.
- Read the absorbance at 340 nm from 300 seconds at 30-second intervals, and work out ΔA/min.
- CK-MB (IU/L) = ΔA/min × 6752.
Every stock code.
CK-MB ships in 3 pack sizes, each with its own stock code.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| CC1-CKM.007 | 20 x 1.1 ml |  |
Datasheet PDF, CC1-CKM.007 |
| CC2-CKM.07U | 10 x 3 ml |  |
Datasheet PDF, CC2-CKM.07U |
| CC2-CKM.07V | 10 x 10 ml |  |
Datasheet PDF, CC2-CKM.07V |
Questions and answers.
CK-MB: what sample does it need?
The datasheet gives the sample as Serum: fresh, clear; heparin plasma may also be used; CK-MB stable 6 to 8 hours at 2 to 8 °C. The sample volume is 50 µl (0.05 ml).
CK-MB: what method does it use?
The method on the datasheet is Immunoinhibition, IFCC; kinetic.
CK-MB: what wavelength is it read at?
According to the datasheet, it is read at 340 nm.
CK-MB: what is the linear range?
The datasheet gives the linear range as Up to 1500 IU/L; dilute higher samples with 0.9% saline.
CK-MB: how should it be stored?
The datasheet gives storage as 2 to 8 °C, until the expiry date on the label.
CK-MB: what pack sizes are available?
It comes in 3 pack sizes, each with its own stock code: 20 x 1.1 ml (CC1-CKM.007), 10 x 3 ml (CC2-CKM.07U), 10 x 10 ml (CC2-CKM.07V).
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