Photographed: the 5 x 25 ml pack, stock code CC2-CLE.005.
2 pack sizes, 125 to 300 ml.
Stock code CC2-CLE.005
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
Intended use: Cholesterol (CHOD-PAP) is a reagent kit for the quantitative estimation of cholesterol in serum or plasma, by the enzymatic method of Allain and Roeschlau. Cholesterol esterase and cholesterol oxidase release hydrogen peroxide, which with peroxidase forms a coloured complex read at 505 nm in proportion to total cholesterol. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 02-2026. The datasheet governs: read it before use, or ask us.
- Method
- Enzymatic CHOD-PAP, end point
- Sample
- Serum
- Sample volume
- 10 µl (0.01 ml)
- Wavelength
- 505 nm (500 to 540 nm, or a green filter)
- Linearity
- Up to 1000 mg/dl
- Storage
- 2 to 8 °C
Full specification19 items
- Category
- Clinical chemistry
- Pack
- 125 to 300 ml
- Pack sizes
- 2 to choose from
- Datasheet
- Published
- Method
- Enzymatic CHOD-PAP, end point
- Sample
- Serum: fresh, clear, fasting; heparin plasma may also be used
- Sample volume
- 10 µl (0.01 ml)
- Reagent volume
- 1.0 ml of working reagent
- Wavelength
- 505 nm (500 to 540 nm, or a green filter)
- Temperature
- 37 °C
- Incubation
- 10 minutes at 37 °C
- Standard
- 200 mg/dl, included
- Reagents
- Enzyme-chromogen powder dissolved in the diluent; lipid clearing factor included
- Working reagent
- Stable for 6 months at 2 to 8 °C
- Colour stability
- 30 minutes, in a cool dark place
- Caution
- Contains sodium azide; do not ingest
- Storage
- 2 to 8 °C, until the expiry date on the label
- Stock codes
- CC2-CLE.005, CC2-CLE.05U
- Made in
- Vadodara, Gujarat, India
Performance2 figures
- Linearity
- Up to 1000 mg/dl; dilute higher samples with 0.9% saline
- Interference
- None from hemoglobin up to 375 mg/dl, free bilirubin up to 7.5 mg/dl, lipemia (as triglycerides) up to 1000 mg/dl
Procedure at a glance5 steps
- Dissolve 1 vial of enzyme-chromogen powder in its diluent (25 ml or 60 ml), mix, and wait 10 minutes.
- Pipette 1.0 ml of working reagent into the blank, standard and test tubes; add 0.01 ml of standard and of sample to their tubes.
- Mix and incubate for 10 minutes at 37 °C.
- Read the test and the standard against the reagent blank at 505 nm.
- Cholesterol (mg/dl) = absorbance of test ÷ absorbance of standard × 200.
Every stock code.
Cholesterol ships in 2 pack sizes, each with its own stock code.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| CC2-CLE.005 | 5 x 25 ml |  |
Datasheet PDF, CC2-CLE.005 |
| CC2-CLE.05U | 5 x 60 ml |  |
Datasheet PDF, CC2-CLE.05U |
Questions and answers.
Cholesterol: what sample does it need?
The datasheet gives the sample as Serum: fresh, clear, fasting; heparin plasma may also be used. The sample volume is 10 µl (0.01 ml).
Cholesterol: what method does it use?
The method on the datasheet is Enzymatic CHOD-PAP, end point.
Cholesterol: what wavelength is it read at?
According to the datasheet, it is read at 505 nm (500 to 540 nm, or a green filter).
Cholesterol: what is the linear range?
The datasheet gives the linear range as Up to 1000 mg/dl; dilute higher samples with 0.9% saline.
Cholesterol: how should it be stored?
The datasheet gives storage as 2 to 8 °C, until the expiry date on the label.
Cholesterol: what pack sizes are available?
It comes in 2 pack sizes, each with its own stock code: 5 x 25 ml (CC2-CLE.005), 5 x 60 ml (CC2-CLE.05U).
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