Product details.

Bicarbonate (PEPC) is a reagent for the in vitro quantitative determination of total CO2 in human serum or plasma. Phosphoenolpyruvate carboxylase uses the sample's bicarbonate to form oxaloacetate, which malate dehydrogenase reduces while oxidising NADH; the fall in absorbance at 380 nm is proportional to the bicarbonate present. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 12-2023. The datasheet governs: read it before use, or ask us.

Method: Enzymatic PEPC
Sample: Serum or heparinised plasma
Sample volume: 0.01 ml
Wavelength: 380 nm (375 to 380 nm)
Linearity: Up to 50 mmol/L at 380 nm
Sensitivity: A change of 0.001 AU equals 0.08 mmol/L

Full specification17 items

Category: Clinical chemistry
Pack: 22 ml
Datasheet: Published
Method: Enzymatic PEPC; end point or fixed time
Sample: Serum or heparinised plasma; free of hemolysis, collected and handled anaerobically
Sample volume: 0.01 ml
Reagent volume: 1.0 ml of working reagent
Wavelength: 380 nm (375 to 380 nm), or 340 nm
Temperature: 37 °C
Incubation: 5 minutes (end point); readings at 30 and 60 seconds (fixed time)
Standard: 25 mmol/L, included
Reagents: Reagent vials reconstituted with the buffer; keep exposure to air to a minimum and do not shake
Working reagent: Stable for at least 15 days at 2 to 8 °C; discard if turbid or below 0.8 absorbance at 380 nm
Caution: Contains sodium azide; flush with plenty of water when disposing
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC1-HCO.036
Made in: Vadodara, Gujarat, India

Performance2 figures

Linearity: Up to 50 mmol/L at 380 nm; up to 40 mmol/L at 340 nm
Sensitivity: A change of 0.001 AU equals 0.08 mmol/L

Procedure at a glance5 steps

Reconstitute each reagent vial with the volume of buffer stated on its label, without shaking.
Pipette 1.0 ml of working reagent into the blank, standard and test tubes; add 0.01 ml of distilled water, standard and sample respectively.
Stopper, mix well and stand for 5 minutes at 37 °C.
Read the blank, standard and test against distilled water at 380 nm.
Bicarbonate (mmol/L) = (blank − sample) ÷ (blank − standard) × 25.