Product details.

Bilirubin (Jendrassik & Grof) is a reagent kit for the quantitative estimation of total and direct bilirubin in serum or plasma. Bilirubin reacts with diazotised sulphanilic acid to form an azo compound whose colour, read at 546 nm, is proportional to the bilirubin present; caffeine accelerates the reaction for total bilirubin. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Jendrassik & Grof, end point
Sample: Serum preferred
Sample volume: 50 µl
Wavelength: 546 nm (530 to 560 nm)
Linearity: > 15 mg/dl
Storage: 2 to 8 °C

Full specification18 items

Category: Clinical chemistry
Pack: 180 ml
Datasheet: Published
Method: Jendrassik & Grof, end point
Sample: Serum preferred; heparin plasma may be used; protect from light, do not use hemolysed samples
Sample volume: 50 µl
Reagent volume: 1.1 ml
Wavelength: 546 nm (530 to 560 nm); bichromatic 546 and 630 nm
Incubation: Total 5 minutes, direct 3 minutes, at room temperature (25 ± 5 °C)
Read within: 10 minutes
Factor: 26.312
Reagents: Bilirubin 1A, 1B, 2 and 3; working solution 1 is equal parts of 1A and 1B
Opened vial: 1-Bilirubin A: 3 months at 2 to 8 °C
Note: Reagent 3 may form crystals when cold; warm to 37 °C to dissolve
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC3-BIG.004
Made in: Vadodara, Gujarat, India

Performance2 figures

Linearity: > 15 mg/dl
Interference: None from hemoglobin up to 50 mg/dl

Procedure at a glance5 steps

Mix equal volumes of 1-Bilirubin A and B to make working solution 1, and bring reagent 3 to room temperature.
Total bilirubin: to 50 µl of sample add 100 µl of working solution 1 and 1.0 ml of solution 3; for the sample blank use solution 2 instead of working solution 1.
Mix, incubate for 5 minutes at room temperature, and read against the sample blank at 546 nm within 10 minutes.
Direct bilirubin: to 50 µl of sample add 1.0 ml of normal saline and 100 µl of working solution 1 (blank: solution 2), and read after 3 minutes.
Bilirubin (mg/dl) = (absorbance of sample − absorbance of sample blank) × 26.312.