Photographed: the 2 x 60 ml pack, stock code CC3-CLD.039.
Supplied as 120 ml.
Stock code CC3-CLD.039
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
Intended use: Chloride (thiocyanate) is a reagent kit for the quantitative estimation of chloride in serum or plasma. Chloride ions displace thiocyanate from mercuric thiocyanate, and the freed thiocyanate forms red-brown ferric thiocyanate, read at 480 nm in proportion to the chloride present. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.
- Method
- Mercuric thiocyanate
- Sample
- Serum
- Sample volume
- 10 µl (0.01 ml)
- Linearity
- 75 to 150 mmol/L
- Storage
- Reagent at room temperature
- Pack
- 120 ml
Full specification16 items
- Category
- Clinical chemistry
- Pack
- 120 ml
- Datasheet
- Published
- Method
- Mercuric thiocyanate, end point
- Sample
- Serum: fresh, clear, unhemolysed; heparinised plasma may be used
- Sample volume
- 10 µl (0.01 ml)
- Reagent volume
- 1.0 ml
- Wavelength
- 480 nm (470 to 510 nm, or a blue-green filter)
- Incubation
- 5 minutes at room temperature (25 to 30 °C)
- Flowcell temperature
- 30 °C
- Standard
- 100 mmol/L, supplied separately
- Colour stability
- 30 minutes
- Note
- Disposable plasticware recommended; decontaminate glassware in 0.1 N HNO3
- Storage
- Reagent at room temperature; standard at 2 to 8 °C
- Stock codes
- CC3-CLD.039
- Made in
- Vadodara, Gujarat, India
Performance1 figure
- Linearity
- 75 to 150 mmol/L; dilute higher samples, use more sample for lower
Procedure at a glance5 steps
- Pipette 1.0 ml of reagent into the blank, standard and test tubes.
- Add 0.01 ml of standard to the standard tube and 0.01 ml of sample to the test tube.
- Mix and incubate for 5 minutes at room temperature (25 to 30 °C).
- Read against the reagent blank at 480 nm.
- Chloride (mmol/L) = absorbance of test ÷ absorbance of standard × 100.
Every stock code.
Chloride ships in one pack size.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| CC3-CLD.039 | 2 x 60 ml |  |
Datasheet PDF, CC3-CLD.039 |
Questions and answers.
Chloride: what sample does it need?
The datasheet gives the sample as Serum: fresh, clear, unhemolysed; heparinised plasma may be used. The sample volume is 10 µl (0.01 ml).
Chloride: what method does it use?
The method on the datasheet is Mercuric thiocyanate, end point.
Chloride: what wavelength is it read at?
According to the datasheet, it is read at 480 nm (470 to 510 nm, or a blue-green filter).
Chloride: what is the linear range?
The datasheet gives the linear range as 75 to 150 mmol/L; dilute higher samples, use more sample for lower.
Chloride: how should it be stored?
The datasheet gives storage as Reagent at room temperature; standard at 2 to 8 °C.
Chloride: what pack sizes are available?
It comes in one pack size: 2 x 60 ml (CC3-CLD.039).
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