Name: Alkaline Phosphatase (DST)
Brand: Reckon Diagnostics
SKU: CC2-ALK.002

Photographed: the 10 x 5 ml pack, stock code CC2-ALK.002.

DST format. 2 pack sizes, 50 to 100 ml.

Choose a pack size

10 x 5 mlCC2-ALK.002 10 x 10 mlCC2-ALK.02U

Stock code CC2-ALK.002

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Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

Alkaline Phosphatase (DST) is a reagent kit for the quantitative estimation of alkaline phosphatase activity in serum or plasma, by a kinetic method formulated to the recommendation of the German Society for Clinical Chemistry (GSCC). Alkaline phosphatase hydrolyses p-nitrophenyl phosphate to p-nitrophenol, and the rate of increase in absorbance at 405 nm is proportional to its activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Kinetic p-NPP
Sample: Serum
Sample volume: 20 µl (0.02 ml)
Wavelength: 405 nm
Linearity: Up to 1500 IU/L
Storage: 2 to 8 °C

Full specification19 items

Category: Clinical chemistry
Format: DST
Pack: 50 to 100 ml
Pack sizes: 2 to choose from
Datasheet: Published
Method: Kinetic p-NPP, DEA buffer (GSCC)
Sample: Serum: fresh, clear, fasting, no hemolysis; heparin plasma may be used, not oxalate, citrate or EDTA
Sample volume: 20 µl (0.02 ml)
Reagent volume: 1.0 ml of buffered substrate
Wavelength: 405 nm
Temperature: 37 °C
Readings: 4, at 30-second intervals after a 30-second delay
Factor: 2713
Reagents: Substrate tablet and buffer; 1 tablet dissolves in the 5 ml or 10 ml buffer bottle
Working reagent: Use the same day, or keep up to 30 days at 2 to 8 °C in the dark bottle provided
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC2-ALK.002, CC2-ALK.02U
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 1500 IU/L; dilute higher samples with 0.9% saline

Procedure at a glance5 steps

Transfer 1 substrate tablet into the buffer bottle (5 ml or 10 ml), mix gently to dissolve, and wait 15 minutes.
Add 0.02 ml of sample to 1.0 ml of buffered substrate and mix.
Read the absorbance at 405 nm at 30, 60, 90 and 120 seconds, at 37 °C.
Work out the mean change in absorbance per minute (ΔA/min).
Alkaline phosphatase (IU/L) = ΔA/min × 2713.