Photographed: the 10 x 5 ml pack, stock code CC2-ALK.002.
DST format. 2 pack sizes, 50 to 100 ml.
Stock code CC2-ALK.002
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
Intended use: Alkaline Phosphatase (DST) is a reagent kit for the quantitative estimation of alkaline phosphatase activity in serum or plasma, by a kinetic method formulated to the recommendation of the German Society for Clinical Chemistry (GSCC). Alkaline phosphatase hydrolyses p-nitrophenyl phosphate to p-nitrophenol, and the rate of increase in absorbance at 405 nm is proportional to its activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.
- Method
- Kinetic p-NPP
- Sample
- Serum
- Sample volume
- 20 µl (0.02 ml)
- Wavelength
- 405 nm
- Linearity
- Up to 1500 IU/L
- Storage
- 2 to 8 °C
Full specification19 items
- Category
- Clinical chemistry
- Format
- DST
- Pack
- 50 to 100 ml
- Pack sizes
- 2 to choose from
- Datasheet
- Published
- Method
- Kinetic p-NPP, DEA buffer (GSCC)
- Sample
- Serum: fresh, clear, fasting, no hemolysis; heparin plasma may be used, not oxalate, citrate or EDTA
- Sample volume
- 20 µl (0.02 ml)
- Reagent volume
- 1.0 ml of buffered substrate
- Wavelength
- 405 nm
- Temperature
- 37 °C
- Readings
- 4, at 30-second intervals after a 30-second delay
- Factor
- 2713
- Reagents
- Substrate tablet and buffer; 1 tablet dissolves in the 5 ml or 10 ml buffer bottle
- Working reagent
- Use the same day, or keep up to 30 days at 2 to 8 °C in the dark bottle provided
- Caution
- Contains sodium azide; do not ingest
- Storage
- 2 to 8 °C, until the expiry date on the label
- Stock codes
- CC2-ALK.002, CC2-ALK.02U
- Made in
- Vadodara, Gujarat, India
Performance1 figure
- Linearity
- Up to 1500 IU/L; dilute higher samples with 0.9% saline
Procedure at a glance5 steps
- Transfer 1 substrate tablet into the buffer bottle (5 ml or 10 ml), mix gently to dissolve, and wait 15 minutes.
- Add 0.02 ml of sample to 1.0 ml of buffered substrate and mix.
- Read the absorbance at 405 nm at 30, 60, 90 and 120 seconds, at 37 °C.
- Work out the mean change in absorbance per minute (ΔA/min).
- Alkaline phosphatase (IU/L) = ΔA/min × 2713.
Every stock code.
Alkaline Phosphatase (DST) ships in 2 pack sizes, each with its own stock code.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| CC2-ALK.002 | 10 x 5 ml |  |
Datasheet PDF, CC2-ALK.002 |
| CC2-ALK.02U | 10 x 10 ml |  |
Datasheet PDF, CC2-ALK.02U |
Questions and answers.
Alkaline Phosphatase (DST): what sample does it need?
The datasheet gives the sample as Serum: fresh, clear, fasting, no hemolysis; heparin plasma may be used, not oxalate, citrate or EDTA. The sample volume is 20 µl (0.02 ml).
Alkaline Phosphatase (DST): what method does it use?
The method on the datasheet is Kinetic p-NPP, DEA buffer (GSCC).
Alkaline Phosphatase (DST): what wavelength is it read at?
According to the datasheet, it is read at 405 nm.
Alkaline Phosphatase (DST): what is the linear range?
The datasheet gives the linear range as Up to 1500 IU/L; dilute higher samples with 0.9% saline.
Alkaline Phosphatase (DST): how should it be stored?
The datasheet gives storage as 2 to 8 °C, until the expiry date on the label.
Alkaline Phosphatase (DST): what pack sizes are available?
It comes in 2 pack sizes, each with its own stock code: 10 x 5 ml (CC2-ALK.002), 10 x 10 ml (CC2-ALK.02U).
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