Photographed: the 20 x 1.1 ml pack, stock code CC1-AMY.003.
Supplied as 22 ml.
Stock code CC1-AMY.003
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
Intended use: α-Amylase is a reagent kit for the quantitative estimation of α-amylase in serum, by a kinetic method using the blocked chromogenic substrate GAL-G2-CNP. α-Amylase breaks the substrate down to release 2-chloro-4-nitrophenol (CNP), measured at 405 nm in proportion to α-amylase activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.
- Method
- Kinetic, GAL-G2-CNP substrate
- Sample
- Serum
- Sample volume
- 50 µl (0.05 ml)
- Wavelength
- 405 nm
- Linearity
- Up to 1200 IU/L
- Storage
- 2 to 8 °C
Full specification17 items
- Category
- Clinical chemistry
- Pack
- 22 ml
- Datasheet
- Published
- Method
- Kinetic, GAL-G2-CNP substrate
- Sample
- Serum; amylase activity in serum is stable for 20 days at 2 to 4 °C
- Sample volume
- 50 µl (0.05 ml)
- Reagent volume
- 1.0 ml of working reagent
- Wavelength
- 405 nm
- Temperature
- 37 °C
- Readings
- 4, at 30-second intervals after a 60-second delay
- Factor
- 1628
- Reagents
- Substrate tablets and buffer; 1 tablet in 1.1 ml of buffer makes the working reagent
- Working reagent
- Stable for 4 weeks at 2 to 8 °C
- Caution
- Contains sodium azide; do not ingest
- Storage
- 2 to 8 °C, until the expiry date on the label
- Stock codes
- CC1-AMY.003
- Made in
- Vadodara, Gujarat, India
Performance1 figure
- Linearity
- Up to 1200 IU/L; dilute higher samples with 0.9% saline
Procedure at a glance5 steps
- Reconstitute 1 substrate tablet with 1.1 ml of buffer, mix gently until dissolved, and wait 5 minutes.
- Add 0.05 ml of sample to 1.0 ml of working reagent and mix immediately.
- Read the absorbance at 405 nm at exactly 60 seconds, then 3 more times at 30-second intervals, at 37 °C.
- Work out the mean change in absorbance per minute (ΔA/min).
- Amylase (IU/L) = ΔA/min × 1628.
Every stock code.
α-Amylase ships in one pack size.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| CC1-AMY.003 | 20 x 1.1 ml |  |
Datasheet PDF, CC1-AMY.003 |
Questions and answers.
α-Amylase: what sample does it need?
The datasheet gives the sample as Serum; amylase activity in serum is stable for 20 days at 2 to 4 °C. The sample volume is 50 µl (0.05 ml).
α-Amylase: what method does it use?
The method on the datasheet is Kinetic, GAL-G2-CNP substrate.
α-Amylase: what wavelength is it read at?
According to the datasheet, it is read at 405 nm.
α-Amylase: what is the linear range?
The datasheet gives the linear range as Up to 1200 IU/L; dilute higher samples with 0.9% saline.
α-Amylase: how should it be stored?
The datasheet gives storage as 2 to 8 °C, until the expiry date on the label.
α-Amylase: what pack sizes are available?
It comes in one pack size: 20 x 1.1 ml (CC1-AMY.003).
More clinical chemistry.
All 50 clinical chemistry reagents →
Reagent bottle
Reagent bottle
Reagent bottle
Reagent bottle
Reagent bottle
Reagent bottle
Reagent bottle
Reagent bottle