Product details.

Albumin (BCG) is a reagent kit for the quantitative estimation of serum albumin. In an acidic medium albumin binds bromocresol green, and the blue-green colour formed, read at 630 nm, is proportional to the albumin present. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Bromocresol green (BCG) dye binding
Sample: Fasting, clear serum preferred
Sample volume: 10 µl (0.01 ml)
Wavelength: 630 nm (600 to 650 nm, or a red filter)
Linearity: Up to 6 g/dl with 1 ml of reagent
Storage: Reagent at room temperature

Full specification14 items

Category: Clinical chemistry
Pack: 240 ml
Datasheet: Published
Method: Bromocresol green (BCG) dye binding, end point
Sample: Fasting, clear serum preferred; plasma may be used
Sample volume: 10 µl (0.01 ml)
Reagent volume: 1.0 ml, or 2.5 ml
Wavelength: 630 nm (600 to 650 nm, or a red filter)
Incubation: 10 minutes at room temperature (not below 25 °C)
Standard: 5 g/dl, supplied separately
Colour stability: 1 hour
Storage: Reagent at room temperature; standard at 2 to 8 °C
Stock codes: CC3-ALN.034
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 6 g/dl with 1 ml of reagent; up to 10 g/dl with 2.5 ml

Procedure at a glance5 steps

Pipette 1.0 ml of reagent into the blank, standard and test tubes.
Add 0.01 ml of standard to the standard tube and 0.01 ml of sample to the test tube.
Mix and stand for 10 minutes at room temperature (not below 25 °C).
Read the test and the standard against the reagent blank at 630 nm.
Albumin (g/dl) = absorbance of test ÷ absorbance of standard × 5.