Drawing, not a photograph. This pack has not been photographed yet; the stock codes and pack sizes below are the specification.
Supplied as 55 ml.
Stock code CC1-ALK.02M
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
Alkaline Phosphatase is a reagent kit for the quantitative estimation of alkaline phosphatase activity in serum or plasma, by a kinetic method formulated to the recommendation of the German Society for Clinical Chemistry (GSCC). Alkaline phosphatase hydrolyses p-nitrophenyl phosphate to p-nitrophenol, and the rate of increase in absorbance at 405 nm is proportional to its activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.
- Method
- Kinetic p-NPP
- Sample
- Serum
- Sample volume
- 20 µl (0.02 ml)
- Wavelength
- 405 nm
- Linearity
- Up to 1500 IU/L
- Storage
- 2 to 8 °C
Full specification17 items
- Category
- Clinical chemistry
- Pack
- 55 ml
- Datasheet
- Published
- Method
- Kinetic p-NPP, DEA buffer (GSCC)
- Sample
- Serum: fresh, clear, fasting, no hemolysis; heparin plasma may be used, not oxalate, citrate or EDTA
- Sample volume
- 20 µl (0.02 ml)
- Reagent volume
- 1.0 ml of buffered substrate
- Wavelength
- 405 nm
- Temperature
- 37 °C
- Readings
- 4, at 30-second intervals after a 30-second delay
- Factor
- 2713
- Reagents
- Substrate tablets and buffer; 1 tablet in 1.1 ml of buffer makes the working substrate
- Working reagent
- Use the same day, or keep up to 10 days at 2 to 8 °C in a dark bottle
- Caution
- Contains sodium azide; do not ingest
- Storage
- 2 to 8 °C, until the expiry date on the label
- Stock codes
- CC1-ALK.02M
- Made in
- Vadodara, Gujarat, India
Performance1 figure
- Linearity
- Up to 1500 IU/L; dilute higher samples with 0.9% saline
Procedure at a glance5 steps
- Dissolve 1 substrate tablet in 1.1 ml of buffer (crush it first to speed this up) and wait 15 minutes.
- Add 0.02 ml of sample to 1.0 ml of buffered substrate and mix.
- Read the absorbance at 405 nm at 30, 60, 90 and 120 seconds, at 37 °C.
- Work out the mean change in absorbance per minute (ΔA/min).
- Alkaline phosphatase (IU/L) = ΔA/min × 2713.
Every stock code.
Alkaline Phosphatase ships in one pack size.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| CC1-ALK.02M | 50 x 1.1 ml | — | Datasheet |
More clinical chemistry.
All 51 in clinical chemistry →
Reagent bottle
Reagent bottle
Reagent bottle
Reagent bottle
Reagent bottle
Reagent bottle
Reagent bottle
Reagent bottle