Name: UIBC
Brand: Reckon Diagnostics
SKU: CC1-UIB.55M

Reagent bottle: no photograph of this pack is published yet

Drawing, not a photograph. This pack has not been photographed yet; the stock codes and pack sizes below are the specification.

Supplied as 20 tests.

Pack size

20 TestCC1-UIB.55M

Stock code CC1-UIB.55M

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Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

UIBC (Ferrozine) is a reagent kit for the quantitative estimation of unsaturated and total iron-binding capacity (UIBC and TIBC) in serum or plasma. A known excess of ferrous iron saturates transferrin in alkaline medium, ferrozine forms a purple complex with the iron left unbound, measured at 560 nm, and the difference from the excess added is the UIBC. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Ferrozine, end point (increase)
Sample: Serum or plasma
Sample volume: 200 µl (0.200 ml)
Wavelength: 560 nm
Linearity: Up to 89 µmol/L (500 µg/dl)
Storage: 2 to 8 °C

Full specification18 items

Category: Clinical chemistry
Pack: 20 tests
Datasheet: Published
Method: Ferrozine, end point (increase)
Sample: Serum or plasma: fresh, clear, no hemolysis; stable 1 day at room temperature, 1 week at 2 to 8 °C
Sample volume: 200 µl (0.200 ml)
Reagent volume: 1.0 ml of working reagent, then 0.05 ml of colour reagent
Wavelength: 560 nm; bichromatic 560 and 630 nm
Temperature: 37 °C
Incubation: 10 minutes at 37 °C
Limitation: Hemolysis gives falsely high results; iron medication affects serum levels
Standard: 80 µmol/L (446 µg/dl), included
Reagents: Buffer, colour and enhancer reagents; 1 vial of enhancer in 1 bottle of buffer, used after 15 minutes
Working reagent: 60 days at 2 to 8 °C
Note: Glassware must be iron-free: soak in 0.1 N HNO3 or 1 N HCl and rinse with deionised water
Storage: 2 to 8 °C, protected from light
Stock codes: CC1-UIB.55M
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 89 µmol/L (500 µg/dl); dilute higher samples with 0.9% saline

Procedure at a glance5 steps

Dissolve 1 vial of 3 UIBC (enhancer) in 1 bottle of 1 UIBC (buffer) and wait 15 minutes.
Pipette 1.0 ml of working reagent into four tubes: blank (0.4 ml of water), standard (0.2 ml of standard + 0.2 ml of water), and sample blank and test (0.2 ml of sample + 0.2 ml of standard each).
Add 0.05 ml of 2 UIBC (colour reagent) to the blank, standard and test, not to the sample blank.
Stand for 10 minutes at 37 °C and read against the reagent blank at 560 nm.
Excess iron (µmol/L) = (test − sample blank) ÷ standard × 80; UIBC = 80 − excess iron; TIBC = serum iron + UIBC.