Urea-GLDH (DST) Reagent, CC2-UAG.19N

Photographed: the 5 x 25 ml pack, stock code CC2-UAG.19N.

DST format. 3 pack sizes, 120 to 300 ml.

Choose a pack size

5 x 25 mlCC2-UAG.19N 2 x 60 mlCC2-UAG.19NU 5 x 60 mlCC2-UAG.19NV

Stock code CC2-UAG.19N

Ask about this product → Datasheet (PDF) (opens in a new tab)

Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

Intended use: Urea-GLDH (DST) is a reagent kit for the quantitative estimation of urea and BUN in serum or plasma, by a UV fixed-time method. Urease releases ammonia from urea, and glutamate dehydrogenase uses it with 2-oxoglutarate to form glutamate while oxidising NADH to NAD; the decrease in absorbance at 340 nm is proportional to urea. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Urease-GLDH, UV fixed time (decreasing)
Sample: Serum
Sample volume: 20 µl (0.02 ml)
Wavelength: 340 nm
Linearity: Up to 300 mg/dl
Storage: 2 to 8 °C

Full specification19 items

Category: Clinical chemistry
Format: DST
Pack: 120 to 300 ml
Pack sizes: 3 to choose from
Datasheet: Published
Method: Urease-GLDH, UV fixed time (decreasing)
Sample: Serum: fresh, fasting, clear, no hemolysis; plasma with heparin, oxalate or citrate may be used
Sample volume: 20 µl (0.02 ml)
Reagent volume: 1.0 ml of working reagent
Wavelength: 340 nm
Temperature: 25 °C or 30 °C
Readings: At 20 and 80 seconds, or at 30 and 90 seconds
Standard: 50 mg/dl, included
Reagents: Enzyme-coenzyme powder dissolved in the urea buffer (25 ml or 60 ml); use after 15 minutes
Working reagent: 4 months at 2 to 8 °C
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC2-UAG.19N, CC2-UAG.19NU, CC2-UAG.19NV
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 300 mg/dl; dilute higher samples 1 + 1 with 0.9% saline

Procedure at a glance5 steps

Dissolve 1 vial of urea powder in its buffer (25 ml or 60 ml) and wait 15 minutes.
Add 0.02 ml of standard or sample to 1.0 ml of working reagent and mix immediately.
Read the absorbance at 340 nm at 20 seconds (AT1) and 80 seconds (AT2), or at 30 and 90 seconds.
Work out ΔA/min = AT1 − AT2 for the standard and for the test.
Urea (mg/dl) = ΔA/min of test × 50 ÷ ΔA/min of standard.

Every stock code.

Urea-GLDH (DST) ships in 3 pack sizes, each with its own stock code.

Stock codes and pack sizes for Urea-GLDH (DST)
Stock code	Pack	Datasheet
CC2-UAG.19N	5 x 25 ml	Datasheet PDF, CC2-UAG.19N
CC2-UAG.19NU	2 x 60 ml	Datasheet PDF, CC2-UAG.19NU
CC2-UAG.19NV	5 x 60 ml	Datasheet PDF, CC2-UAG.19NV

Questions and answers.

Urea-GLDH (DST): what sample does it need?

The datasheet gives the sample as Serum: fresh, fasting, clear, no hemolysis; plasma with heparin, oxalate or citrate may be used. The sample volume is 20 µl (0.02 ml).

Urea-GLDH (DST): what method does it use?

The method on the datasheet is Urease-GLDH, UV fixed time (decreasing).

Urea-GLDH (DST): what wavelength is it read at?

According to the datasheet, it is read at 340 nm.

Urea-GLDH (DST): what is the linear range?

The datasheet gives the linear range as Up to 300 mg/dl; dilute higher samples 1 + 1 with 0.9% saline.

Urea-GLDH (DST): how should it be stored?

The datasheet gives storage as 2 to 8 °C, until the expiry date on the label.

Urea-GLDH (DST): what pack sizes are available?

It comes in 3 pack sizes, each with its own stock code: 5 x 25 ml (CC2-UAG.19N), 2 x 60 ml (CC2-UAG.19NU), 5 x 60 ml (CC2-UAG.19NV).