Product details.

Urea (Berthelot) is a reagent kit for the quantitative estimation of urea and BUN in serum or plasma. Urease breaks urea down into ammonia and carbon dioxide; in alkaline medium the ammonia reacts with hypochlorite and salicylate, catalysed by nitroprusside, to form a dicarboxyindophenol measured at 578 nm. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 07-2025. The datasheet governs: read it before use, or ask us.

Method: Urease-Berthelot, end point
Sample: Serum
Sample volume: 10 µl (0.01 ml)
Wavelength: 578 nm (570 to 620 nm, or a red filter)
Linearity: Up to 350 mg/dl
Storage: 2 to 8 °C

Full specification20 items

Category: Clinical chemistry
Pack: 50 to 300 ml
Pack sizes: 3 to choose from
Datasheet: Published
Method: Urease-Berthelot, end point
Sample: Serum, or plasma with heparin, oxalate or citrate; urine diluted in normal saline
Sample volume: 10 µl (0.01 ml)
Reagent volume: 1.0 ml of working enzyme reagent + 1.0 ml of colour developing reagent
Wavelength: 578 nm (570 to 620 nm, or a red filter)
Temperature: 37 °C
Incubation: 5 + 5 minutes at 37 °C, or 10 + 10 minutes at room temperature (25 to 30 °C)
Standard: 40 mg/dl, included
Colour stability: 1 hour
Reagents: Enzyme reconstituted with deionised water (10 ml or 60 ml), used after 10 minutes; colour developing reagent ready to use
Working reagent: Enzyme: 6 months at 2 to 8 °C, protected from light; colour reagent: 3 months once opened
Note: Reagent 2 may crystallise; 5 minutes at 37 °C dissolves the crystals
Caution: Colour developing reagent is corrosive; do not pipette by mouth; contains sodium azide
Storage: 2 to 8 °C, until the expiry date on the kit label
Stock codes: CC2-UAB.019, CC2-UAB.19U, CC2-UAB.19V
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 350 mg/dl; dilute higher samples 1 + 1 with 0.9% saline

Procedure at a glance5 steps

Dissolve the enzyme vial in deionised water (10 ml or 60 ml) and wait 10 minutes.
Pipette 1.0 ml of working enzyme reagent into the blank, standard and test tubes with 0.01 ml of distilled water, standard and sample respectively.
Mix and incubate for 10 minutes at room temperature (25 to 30 °C), or 5 minutes at 37 °C.
Add 1.0 ml of colour developing reagent, mix, incubate again for the same time, and read the test and standard against the reagent blank at 578 nm.
Urea (mg/dl) = absorbance of test ÷ absorbance of standard × 40; BUN (mg/dl) = 0.467 × urea.