Product details.

Triglycerides-L (Single Liquid) is a ready-to-use reagent for the quantitative estimation of triglycerides in serum or plasma, by the GPO method. Lipoprotein lipase releases glycerol, which glycerol kinase and glycerol-3-phosphate oxidase convert to hydrogen peroxide; peroxidase then forms a pink quinoneimine dye with 4-aminoantipyrine and chlorophenol, read at 520 nm. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Enzymatic GPO, end point
Sample: Serum
Sample volume: 20 µl (0.02 ml)
Wavelength: 520 nm (500 to 550 nm, or a green filter)
Linearity: Up to 800 mg/dl
Storage: 2 to 8 °C

Full specification19 items

Category: Clinical chemistry
Pack: 125 to 300 ml
Pack sizes: 2 to choose from
Datasheet: Published
Method: Enzymatic GPO, end point
Sample: Serum: fresh, clear, fasting, no hemolysis; heparin or citrate plasma may be used
Sample volume: 20 µl (0.02 ml)
Reagent volume: 1.0 ml
Wavelength: 520 nm (500 to 550 nm, or a green filter)
Temperature: 37 °C
Incubation: 10 minutes at 37 °C
Standard: 200 mg/dl, included
Reagents: Single liquid reagent, ready to use
Shelf life: 18 months at 2 to 8 °C, protected from light
Note: Serum reads about 5 mg/dl higher than plasma
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC3-TGS.18N, CC3-TGS.18NU
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 800 mg/dl; dilute higher samples with 0.9% saline

Procedure at a glance4 steps

Pipette 1.0 ml of reagent into the blank, standard and test tubes; add 0.020 ml of standard and of sample to their tubes.
Mix and incubate at 37 °C for 10 minutes.
Read the test and the standard against the reagent blank at 520 nm.
Triglycerides (mg/dl) = absorbance of test ÷ absorbance of standard × 200; multiply by 0.0114 for mmol/L.