Photographed: the 5 x 25 ml pack, stock code CC3-TGS.18N.
2 pack sizes, 125 to 300 ml.
Stock code CC3-TGS.18N
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
Intended use: Triglycerides-L (Single Liquid) is a ready-to-use reagent for the quantitative estimation of triglycerides in serum or plasma, by the GPO method. Lipoprotein lipase releases glycerol, which glycerol kinase and glycerol-3-phosphate oxidase convert to hydrogen peroxide; peroxidase then forms a pink quinoneimine dye with 4-aminoantipyrine and chlorophenol, read at 520 nm. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.
- Method
- Enzymatic GPO, end point
- Sample
- Serum
- Sample volume
- 20 µl (0.02 ml)
- Wavelength
- 520 nm (500 to 550 nm, or a green filter)
- Linearity
- Up to 800 mg/dl
- Storage
- 2 to 8 °C
Full specification19 items
- Category
- Clinical chemistry
- Pack
- 125 to 300 ml
- Pack sizes
- 2 to choose from
- Datasheet
- Published
- Method
- Enzymatic GPO, end point
- Sample
- Serum: fresh, clear, fasting, no hemolysis; heparin or citrate plasma may be used
- Sample volume
- 20 µl (0.02 ml)
- Reagent volume
- 1.0 ml
- Wavelength
- 520 nm (500 to 550 nm, or a green filter)
- Temperature
- 37 °C
- Incubation
- 10 minutes at 37 °C
- Standard
- 200 mg/dl, included
- Reagents
- Single liquid reagent, ready to use
- Shelf life
- 18 months at 2 to 8 °C, protected from light
- Note
- Serum reads about 5 mg/dl higher than plasma
- Caution
- Contains sodium azide; do not ingest
- Storage
- 2 to 8 °C, until the expiry date on the label
- Stock codes
- CC3-TGS.18N, CC3-TGS.18NU
- Made in
- Vadodara, Gujarat, India
Performance1 figure
- Linearity
- Up to 800 mg/dl; dilute higher samples with 0.9% saline
Procedure at a glance4 steps
- Pipette 1.0 ml of reagent into the blank, standard and test tubes; add 0.020 ml of standard and of sample to their tubes.
- Mix and incubate at 37 °C for 10 minutes.
- Read the test and the standard against the reagent blank at 520 nm.
- Triglycerides (mg/dl) = absorbance of test ÷ absorbance of standard × 200; multiply by 0.0114 for mmol/L.
Every stock code.
Triglycerides-L ships in 2 pack sizes, each with its own stock code.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| CC3-TGS.18N | 5 x 25 ml |  |
Datasheet PDF, CC3-TGS.18N |
| CC3-TGS.18NU | 5 x 60 ml |  |
Datasheet PDF, CC3-TGS.18NU |
Questions and answers.
Triglycerides-L: what sample does it need?
The datasheet gives the sample as Serum: fresh, clear, fasting, no hemolysis; heparin or citrate plasma may be used. The sample volume is 20 µl (0.02 ml).
Triglycerides-L: what method does it use?
The method on the datasheet is Enzymatic GPO, end point.
Triglycerides-L: what wavelength is it read at?
According to the datasheet, it is read at 520 nm (500 to 550 nm, or a green filter).
Triglycerides-L: what is the linear range?
The datasheet gives the linear range as Up to 800 mg/dl; dilute higher samples with 0.9% saline.
Triglycerides-L: how should it be stored?
The datasheet gives storage as 2 to 8 °C, until the expiry date on the label.
Triglycerides-L: what pack sizes are available?
It comes in 2 pack sizes, each with its own stock code: 5 x 25 ml (CC3-TGS.18N), 5 x 60 ml (CC3-TGS.18NU).
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