Product details.

Lipase (turbidimetric) is a reagent for the in vitro quantitative determination of lipase (triacylglycerolase) in human serum, by the method of Ziegenhorn et al. Lipase, with colipase, hydrolyses triolein to glycerides and fatty acids, and the rate of decrease in turbidity at 340 nm is proportional to lipase activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 02-2026. The datasheet governs: read it before use, or ask us.

Method: Turbidimetric
Sample: Non-hemolysed serum
Wavelength: 340 nm
Linearity: Up to 700 U/L
Storage: 2 to 8 °C
Pack: 16.5 ml

Full specification18 items

Category: Clinical chemistry
Pack: 16.5 ml
Datasheet: Published
Method: Turbidimetric, fixed time (decreasing)
Sample: Non-hemolysed serum; stable 1 week at 18 to 25 °C, 3 weeks at 2 to 8 °C
Sample volume: 0.04 ml, or 0.02 ml in the half-volume procedure
Reagent volume: 1.0 ml of working reagent, or 0.5 ml
Wavelength: 340 nm
Temperature: 37 °C
Readings: At 240 seconds and 540 seconds
Calibration: Lipase calibrator included; reconstitute with 2.0 ml of diluent
Working reagent: 14 days at 2 to 8 °C
Calibrator stability: 7 days at 2 to 8 °C; 4 weeks at −20 °C
Note: Avoid cross-contamination from cholesterol, triglyceride, HDL and LDL reagents
Caution: Avoid ingestion and contact with skin, mouth and eyes
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC1-LPS.015
Made in: Vadodara, Gujarat, India

Performance2 figures

Linearity: Up to 700 U/L
Interference: None from hemoglobin up to 124 µmol/L, bilirubin up to 342 µmol/L

Procedure at a glance5 steps

Dissolve 1 vial of 1 Lipase with 1.1 ml of buffer; reconstitute the calibrator with 2.0 ml of diluent.
Pipette 1.0 ml of working reagent into the calibrator and test tubes; add 0.04 ml of calibrator and of sample to their tubes.
Mix and read the absorbance at 340 nm at exactly 240 seconds and again at 540 seconds.
Work out ΔA = A1 − A2.
Lipase (U/L) = ΔA of sample ÷ ΔA of calibrator × calibrator value.