Name: SGOT (DST)
Brand: Reckon Diagnostics
SKU: CC2-AST.16N

Photographed: the 5 x 25 ml pack, stock code CC2-AST.16N.

DST format. 2 pack sizes, 125 to 300 ml.

Choose a pack size

5 x 25 mlCC2-AST.16N 5 x 60 mlCC2-AST.16NU

Stock code CC2-AST.16N

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Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

SGOT (DST) is a reagent kit for the quantitative estimation of glutamate oxaloacetate transaminase (AST) activity in serum or plasma, by the kinetic procedure recommended by the IFCC. AST forms oxaloacetate from L-aspartate and α-ketoglutarate; malate dehydrogenase reduces it while oxidising NADH, and the fall in absorbance at 340 nm is proportional to AST activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 01-2026. The datasheet governs: read it before use, or ask us.

Method: Kinetic, IFCC (decreasing absorbance)
Sample: Serum
Sample volume: 100 µl (0.1 ml)
Wavelength: 340 nm
Linearity: Up to 500 IU/L
Storage: 2 to 8 °C

Full specification19 items

Category: Clinical chemistry
Format: DST
Pack: 125 to 300 ml
Pack sizes: 2 to choose from
Datasheet: Published
Method: Kinetic, IFCC (decreasing absorbance)
Sample: Serum: fresh, clear, fasting, no hemolysis; heparin or EDTA plasma may be used
Sample volume: 100 µl (0.1 ml)
Reagent volume: 1.0 ml of working reagent
Wavelength: 340 nm
Temperature: 37 °C
Readings: 4, at 30-second intervals after a 60-second delay
Factor: 1746
Reagents: Enzyme-coenzyme powder dissolved in the buffer substrate (25 ml or 60 ml); use after 5 minutes
Working reagent: 4 months at 2 to 8 °C
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC2-AST.16N, CC2-AST.16NU
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 500 IU/L; dilute higher samples with 0.9% saline

Procedure at a glance5 steps

Dissolve 1 vial of SGOT powder in its buffer (25 ml or 60 ml) and wait 5 minutes.
Add 0.1 ml of sample to 1.0 ml of working reagent and mix.
After 60 seconds at 37 °C, read the absorbance at 340 nm every 30 seconds for 2 minutes.
Work out the mean change in absorbance per minute (ΔA/min).
SGOT (IU/L) = ΔA/min × 1746.