SGOT-L (R1-R2) Reagent, CC3-AST.16M

Photographed: the 2 x 25 ml pack, stock code CC3-AST.16M.

R1-R2 format. 2 pack sizes, 50 to 100 ml.

Choose a pack size

2 x 25 mlCC3-AST.16M 4 x 25 mlCC3-AST.16MU

Stock code CC3-AST.16M

Ask about this product → Datasheet (PDF) (opens in a new tab)

Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

Intended use: SGOT-L (R1-R2) is a liquid reagent kit for the quantitative estimation of glutamate oxaloacetate transaminase (AST) activity in serum or plasma, by the kinetic procedure recommended by the IFCC. AST forms oxaloacetate from L-aspartate and α-ketoglutarate; malate dehydrogenase reduces it while oxidising NADH, and the fall in absorbance at 340 nm is proportional to AST activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Kinetic, IFCC (decreasing absorbance)
Sample: Serum
Sample volume: 50 µl (0.05 ml)
Wavelength: 340 nm
Linearity: Up to 500 IU/L
Storage: 2 to 8 °C

Full specification19 items

Category: Clinical chemistry
Format: R1-R2
Pack: 50 to 100 ml
Pack sizes: 2 to choose from
Datasheet: Published
Method: Kinetic, IFCC (decreasing absorbance)
Sample: Serum: fresh, clear, fasting, no hemolysis; heparin or EDTA plasma may be used
Sample volume: 50 µl (0.05 ml)
Reagent volume: 1.0 ml of working reagent, or 0.8 ml of R1 + 0.2 ml of R2
Wavelength: 340 nm
Temperature: 37 °C
Readings: 4, at 30-second intervals after a 60-second delay
Factor: 3376
Reagents: R1 (enzyme) and R2 (substrate), liquid; working reagent is 4 parts R1 to 1 part R2
Working reagent: 30 days at 2 to 8 °C
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC3-AST.16M, CC3-AST.16MU
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 500 IU/L; dilute higher samples with 0.9% saline

Procedure at a glance5 steps

Pour 1 bottle of R2 into the R1 bottle and wait 2 minutes, or mix 4 parts of R1 with 1 part of R2 as needed.
Add 0.05 ml of sample to 1.0 ml of working reagent (or to 0.8 ml of R1 and 0.2 ml of R2) and mix.
After 60 seconds at 37 °C, read the absorbance at 340 nm every 30 seconds for 2 minutes.
Work out the mean change in absorbance per minute (ΔA/min).
SGOT (IU/L) = ΔA/min × 3376.

Every stock code.

SGOT-L (R1-R2) ships in 2 pack sizes, each with its own stock code.

Stock codes and pack sizes for SGOT-L (R1-R2)
Stock code	Pack	Photograph	Datasheet
CC3-AST.16M	2 x 25 ml		Datasheet PDF, CC3-AST.16M
CC3-AST.16MU	4 x 25 ml		Datasheet PDF, CC3-AST.16MU

Questions and answers.

SGOT-L (R1-R2): what sample does it need?

The datasheet gives the sample as Serum: fresh, clear, fasting, no hemolysis; heparin or EDTA plasma may be used. The sample volume is 50 µl (0.05 ml).

SGOT-L (R1-R2): what method does it use?

The method on the datasheet is Kinetic, IFCC (decreasing absorbance).

SGOT-L (R1-R2): what wavelength is it read at?

According to the datasheet, it is read at 340 nm.

SGOT-L (R1-R2): what is the linear range?

The datasheet gives the linear range as Up to 500 IU/L; dilute higher samples with 0.9% saline.

SGOT-L (R1-R2): how should it be stored?

The datasheet gives storage as 2 to 8 °C, until the expiry date on the label.

SGOT-L (R1-R2): what pack sizes are available?

It comes in 2 pack sizes, each with its own stock code: 2 x 25 ml (CC3-AST.16M), 4 x 25 ml (CC3-AST.16MU).