Name: SGPT-L (R1-R2)
Brand: Reckon Diagnostics
SKU: CC3-ALT.17M

Photographed: the 2 x 25 ml pack, stock code CC3-ALT.17M.

R1-R2 format. 2 pack sizes, 50 to 100 ml.

Choose a pack size

2 x 25 mlCC3-ALT.17M 4 x 25 mlCC3-ALT.17MU

Stock code CC3-ALT.17M

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Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

SGPT-L (R1-R2) is a liquid reagent kit for the quantitative estimation of glutamate pyruvate transaminase (ALT) activity in serum or plasma, by the kinetic procedure recommended by the IFCC. ALT forms pyruvate from L-alanine and α-ketoglutarate; lactate dehydrogenase reduces it while oxidising NADH, and the fall in absorbance at 340 nm is proportional to ALT activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Kinetic, IFCC (decreasing absorbance)
Sample: Serum
Sample volume: 50 µl (0.05 ml)
Wavelength: 340 nm
Linearity: Up to 500 IU/L
Storage: 2 to 8 °C

Full specification19 items

Category: Clinical chemistry
Format: R1-R2
Pack: 50 to 100 ml
Pack sizes: 2 to choose from
Datasheet: Published
Method: Kinetic, IFCC (decreasing absorbance)
Sample: Serum: fresh, clear, fasting, no hemolysis; heparin or EDTA plasma may be used
Sample volume: 50 µl (0.05 ml)
Reagent volume: 1.0 ml of working reagent, or 0.8 ml of R1 + 0.2 ml of R2
Wavelength: 340 nm
Temperature: 37 °C
Readings: 4, at 30-second intervals after a 60-second delay
Factor: 3376
Reagents: R1 (enzyme) and R2 (substrate), liquid; working reagent is 4 parts R1 to 1 part R2
Working reagent: 30 days at 2 to 8 °C
Caution: Contains sodium azide; do not ingest
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC3-ALT.17M, CC3-ALT.17MU
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 500 IU/L; dilute higher samples with 0.9% saline

Procedure at a glance5 steps

Pour 1 bottle of R2 into the R1 bottle and wait 2 minutes, or mix 4 parts of R1 with 1 part of R2 as needed.
Add 0.05 ml of sample to 1.0 ml of working reagent (or to 0.8 ml of R1 and 0.2 ml of R2) and mix.
After 60 seconds at 37 °C, read the absorbance at 340 nm every 30 seconds for 2 minutes.
Work out the mean change in absorbance per minute (ΔA/min).
SGPT (IU/L) = ΔA/min × 3376.