Product details.

Total Protein (Biuret) is a reagent kit for the quantitative estimation of total protein in serum or plasma. In an alkaline medium, protein reacts with the copper in the biuret reagent to form a blue-violet complex, read at 540 nm in proportion to protein concentration. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Biuret, end point
Sample: Serum
Linearity: Up to 10 g/dl
Storage: Reagent at room temperature
Pack: 240 ml
Datasheet: Published

Full specification17 items

Category: Clinical chemistry
Pack: 240 ml
Datasheet: Published
Method: Biuret, end point
Sample: Serum: fresh, clear, fasting, no hemolysis; plasma with heparin, oxalate, citrate, fluoride or EDTA may be used
Sample volume: 0.05 ml (2.5 ml procedure) or 0.02 ml (1 ml procedure)
Reagent volume: 2.5 ml or 1.0 ml
Wavelength: 540 nm (530 to 570 nm, or a green-yellow filter)
Temperature: 30 °C
Incubation: 10 minutes at room temperature
Standard: 5 g/dl, supplied separately
Colour stability: 1 hour
Reagents: Biuret reagent, liquid, ready to use
Caution: Contains sodium azide; protect eyes from splashes
Storage: Reagent at room temperature; standard at 2 to 8 °C
Stock codes: CC3-TOP.045
Made in: Vadodara, Gujarat, India

Performance2 figures

Linearity: Up to 10 g/dl; dilute higher samples with 0.9% saline
Interference: None from moderate hemoglobin, bilirubin, salicylates and lipids; severely lipemic or icteric samples need a sample blank

Procedure at a glance4 steps

Pipette 2.5 ml of reagent into the blank, standard and test tubes; add 0.05 ml of distilled water, standard and sample respectively (or 1.0 ml and 0.02 ml in the 1 ml procedure).
Mix and stand for 10 minutes at room temperature.
Read the test and the standard against the reagent blank at 540 nm.
Total protein (g/dl) = absorbance of test ÷ absorbance of standard × 5; albumin/globulin ratio = albumin ÷ (total protein − albumin).