Lipase-L (Single Liquid) Reagent, CC3-LPS.15M

Lipase-L (Single Liquid), 4 x 5 ml, view 2

Photographed: the 4 x 5 ml pack, stock code CC3-LPS.15M.

Single liquid format. Supplied as 20 ml.

Pack size

4 x 5 mlCC3-LPS.15M

Stock code CC3-LPS.15M

Ask about this product → Datasheet (PDF) (opens in a new tab)

Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

Intended use: Lipase-L (Single Liquid) is a ready-to-use reagent for the in vitro quantitative determination of lipase (triacylglycerolase) in human serum, by the fixed-time method of Ziegenhorn et al. Lipase, with colipase, hydrolyses triolein to glycerides and fatty acids, and the rate of decrease in turbidity at 340 nm is proportional to lipase activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Turbidimetric
Sample: Non-hemolysed serum
Wavelength: 340 nm
Linearity: Up to 700 U/L
Storage: 2 to 8 °C
Format: Single liquid

Full specification19 items

Category: Clinical chemistry
Format: SINGLE LIQUID
Pack: 20 ml
Datasheet: Published
Method: Turbidimetric, fixed time (decreasing)
Sample: Non-hemolysed serum; stable 1 week at 18 to 25 °C, 3 weeks at 2 to 8 °C
Sample volume: 0.04 ml, or 0.02 ml in the half-volume procedure
Reagent volume: 1.0 ml, or 0.5 ml
Wavelength: 340 nm
Temperature: 37 °C
Readings: At 240 seconds and 540 seconds
Calibration: Lipase calibrator included; reconstitute with 1 ml of diluent
Reagents: Single liquid reagent, ready to use
Calibrator stability: 7 days at 2 to 8 °C; 4 weeks at −20 °C
Note: Keep cholesterol and triglyceride reagents away: they contain lipase and esterase
Caution: Avoid ingestion and contact with skin, mouth and eyes
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC3-LPS.15M
Made in: Vadodara, Gujarat, India

Performance2 figures

Linearity: Up to 700 U/L
Interference: None from hemoglobin up to 124 µmol/L, bilirubin up to 342 µmol/L

Procedure at a glance5 steps

Reconstitute the lipase calibrator with 1 ml of diluent.
Pipette 1.0 ml of reagent into the calibrator and test tubes; add 0.04 ml of calibrator and of sample to their tubes.
Mix and read the absorbance at 340 nm at exactly 240 seconds and again at 540 seconds.
Work out ΔA = A1 − A2.
Lipase (U/L) = ΔA of sample ÷ ΔA of calibrator × calibrator value.

Every stock code.

Lipase-L (Single Liquid) ships in one pack size.

Stock codes and pack sizes for Lipase-L (Single Liquid)
Stock code	Pack	Photograph	Datasheet
CC3-LPS.15M	4 x 5 ml		Datasheet PDF, CC3-LPS.15M

Questions and answers.

Lipase-L (Single Liquid): what sample does it need?

The datasheet gives the sample as Non-hemolysed serum; stable 1 week at 18 to 25 °C, 3 weeks at 2 to 8 °C. The sample volume is 0.04 ml, or 0.02 ml in the half-volume procedure.

Lipase-L (Single Liquid): what method does it use?

The method on the datasheet is Turbidimetric, fixed time (decreasing).

Lipase-L (Single Liquid): what wavelength is it read at?

According to the datasheet, it is read at 340 nm.

Lipase-L (Single Liquid): what is the linear range?

The datasheet gives the linear range as Up to 700 U/L.

Lipase-L (Single Liquid): how should it be stored?

The datasheet gives storage as 2 to 8 °C, until the expiry date on the label.

Lipase-L (Single Liquid): what pack sizes are available?

It comes in one pack size: 4 x 5 ml (CC3-LPS.15M).