Product details.

Magnesium-L (R1-R2) is a reagent for the in vitro quantitative determination of magnesium in human serum, plasma and urine. In alkaline solution magnesium forms a purple complex with calmagite dye, and a chelating agent and detergent in the reagent remove interference from calcium and proteins. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 05-2023. The datasheet governs: read it before use, or ask us.

Method: Calmagite, end point
Sample: Serum or heparinised plasma
Sample volume: 10 µl (0.01 ml)
Wavelength: 520 nm
Linearity: Up to 4.5 mg/dl
Storage: 2 to 8 °C

Full specification19 items

Category: Clinical chemistry
Format: R1-R2
Pack: 20 tests
Datasheet: Published
Method: Calmagite, end point
Sample: Serum or heparinised plasma, not citrate, oxalate or EDTA; 24-hour urine acidified to pH 2 to 3 and diluted 1 + 3
Sample volume: 10 µl (0.01 ml)
Reagent volume: 1.0 ml of working reagent
Wavelength: 520 nm
Temperature: 30 °C or 37 °C
Incubation: 5 minutes
Limitation: Hemolysis gives falsely high results; calcium at 7.5 mmol/L interferes
Standard: 2 mg/dl, included
Reagents: Alkali vials, each with 0.5 ml of dye added
Working reagent: 21 days at 2 to 8 °C
Note: Use disposable plastic tubes and tips; soak any glassware in 1 N HCl
Storage: 2 to 8 °C, until the expiry date on the label
Stock codes: CC1-MAG.056
Made in: Vadodara, Gujarat, India

Performance1 figure

Linearity: Up to 4.5 mg/dl; dilute higher samples with saline

Procedure at a glance5 steps

Add 0.5 ml of 2 Magnesium (dye) to a vial of 1 Magnesium (alkali).
Pipette 1.0 ml of working reagent into the blank, standard and sample tubes; add 0.01 ml of standard and of sample to their tubes.
Mix and stand for 5 minutes.
Read the standard and the sample at 520 nm against distilled water.
Magnesium (mg/dl) = absorbance of sample ÷ absorbance of standard × 2; multiply by 0.412 for mmol/L, and by 4 for diluted urine.