LDL-Direct Reagent, CC3-LDL.022 | Reckon Diagnostics

LDL-Direct, 20 ml / 50 Test with calibrator

LDL-Direct, 20 ml / 50 Test with calibrator, view 2

Photographed: the 20 ml / 50 Test with calibrator pack, stock code CC3-LDL.022.

4 pack sizes, 50 to 200 tests · 56 ml.

Choose a pack size

20 ml / 50 Test with calibratorCC3-LDL.022 40 ml / 100 Test with calibratorCC3-LDL.22M 80 ml / 200 Test with calibratorCC3-LDL.22MU R1 4 x 10 ml + R2 4 x 4 ml (system pack for ReDi 120/240)SYT-LDL.022

Stock code CC3-LDL.022

Ask about this product → Datasheet (PDF) (opens in a new tab)

Supplied by Reckon Diagnostics, Vadodara
Datasheet published: see every stock code below

Product details.

Intended use: LDL-Direct (Selective Detergent) is a two-reagent assay for the quantitative determination of LDL cholesterol, with no pre-treatment or centrifugation. The first step eliminates cholesterol from non-LDL lipoproteins; the second measures LDL cholesterol enzymatically, the colour formed being proportional to its concentration. Supplied by Reckon Diagnostics, Vadodara.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Selective detergent
Sample: Serum, tested without delay after separation
Sample volume: 5 µl
Wavelength: 578 nm
Linearity: Up to 610 mg/dl
Storage: 2 to 8 °C

Full specification18 items

Category: Clinical chemistry
Pack: 50 to 200 tests · 56 ml
Pack sizes: 4 to choose from
Datasheet: Published
Method: Selective detergent, fixed time; no pre-treatment
Sample: Serum, tested without delay after separation; stable 7 days at 2 to 8 °C; avoid repeated freezing
Sample volume: 5 µl
Reagent volume: 375 µl of R1 + 125 µl of R2
Wavelength: 578 nm
Temperature: 37 °C
Incubation: 5 minutes with R1, then 5 minutes after R2
Calibration: HDL/LDL calibrator included (lyophilised human serum)
Reagents: R1 and R2, ready to use
Opened vial: R1 and R2: 4 weeks at 2 to 8 °C
Discard if: Particles or turbidity appear
Storage: 2 to 8 °C, tightly closed, until the expiry date
Stock codes: CC3-LDL.022, CC3-LDL.22M, CC3-LDL.22MU, SYT-LDL.022
Supplied by: Reckon Diagnostics, Vadodara

Performance3 figures

Linearity: Up to 610 mg/dl; dilute higher samples with isotonic saline
Precision: Intra-assay CV 0.5 to 0.8%; inter-assay CV 1.1 to 1.5%
Interference: None up to ascorbic acid 50 mg/dl, hemoglobin 500 mg/dl, bilirubin 30 mg/dl

Procedure at a glance4 steps

Mix 5 µl of calibrator or sample with 375 µl of R1 and incubate for 5 minutes at 37 °C.
Add 125 µl of R2, mix, and read absorbance A1 after 5 seconds.
Incubate for 5 minutes at 37 °C and read absorbance A2 at 578 nm.
LDL cholesterol (mg/dl) = (A2 − A1) of sample ÷ (A2 − A1) of calibrator × calibrator value.

Every stock code.

LDL-Direct ships in 4 pack sizes, each with its own stock code.

Stock codes and pack sizes for LDL-Direct
Stock code	Pack	Photograph	Datasheet
CC3-LDL.022	20 ml / 50 Test with calibrator		Datasheet PDF, CC3-LDL.022
CC3-LDL.22M	40 ml / 100 Test with calibrator		Datasheet PDF, CC3-LDL.22M
CC3-LDL.22MU	80 ml / 200 Test with calibrator		Datasheet PDF, CC3-LDL.22MU
SYT-LDL.022	R1 4 x 10 ml + R2 4 x 4 ml (system pack for ReDi 120/240)	–	–

Questions and answers.

LDL-Direct: what sample does it need?

The datasheet gives the sample as Serum, tested without delay after separation; stable 7 days at 2 to 8 °C; avoid repeated freezing. The sample volume is 5 µl.

LDL-Direct: what method does it use?

The method on the datasheet is Selective detergent, fixed time; no pre-treatment.

LDL-Direct: what wavelength is it read at?

According to the datasheet, it is read at 578 nm.

LDL-Direct: what is the linear range?

The datasheet gives the linear range as Up to 610 mg/dl; dilute higher samples with isotonic saline.

LDL-Direct: how should it be stored?

The datasheet gives storage as 2 to 8 °C, tightly closed, until the expiry date.

LDL-Direct: what pack sizes are available?

It comes in 4 pack sizes, each with its own stock code: 20 ml / 50 Test with calibrator (CC3-LDL.022), 40 ml / 100 Test with calibrator (CC3-LDL.22M), 80 ml / 200 Test with calibrator (CC3-LDL.22MU), R1 4 x 10 ml + R2 4 x 4 ml (system pack for ReDi 120/240) (SYT-LDL.022).