Drawing, not a photograph. This pack has not been photographed yet; the stock codes and pack sizes below are the specification.
2 pack sizes, 22 to 60 ml.
Stock code CC1-LDK.014
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
LDH (L to P, kinetic) is a reagent kit for the quantitative estimation of lactate dehydrogenase activity in serum or plasma, based on the procedure of Gay, Bowers and McComb. LDH oxidises lactate to pyruvate while reducing NAD to NADH, and the rise in absorbance at 340 nm is proportional to LDH activity. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.
- Method
- Kinetic, lactate to pyruvate (L to P)
- Sample
- Serum
- Sample volume
- 50 µl (0.05 ml)
- Wavelength
- 340 nm
- Linearity
- Up to 2000 IU/L
- Storage
- 2 to 8 °C
Full specification18 items
- Category
- Clinical chemistry
- Pack
- 22 to 60 ml
- Pack sizes
- 2 to choose from
- Datasheet
- Published
- Method
- Kinetic, lactate to pyruvate (L to P)
- Sample
- Serum: fresh, clear, no hemolysis
- Sample volume
- 50 µl (0.05 ml)
- Reagent volume
- 1.0 ml of working reagent
- Wavelength
- 340 nm
- Temperature
- 37 °C
- Readings
- 4, at 30-second intervals after a 60-second delay
- Factor
- 3376
- Reagents
- Coenzyme vials reconstituted with buffer (1.1 ml or 3 ml); use after 5 minutes
- Working reagent
- 30 days at 2 to 8 °C
- Caution
- Contains sodium azide; do not ingest
- Storage
- 2 to 8 °C, until the expiry date on the label
- Stock codes
- CC1-LDK.014, CC2-LDK.14U
- Made in
- Vadodara, Gujarat, India
Performance1 figure
- Linearity
- Up to 2000 IU/L; dilute higher samples with 0.9% saline
Procedure at a glance5 steps
- Reconstitute 1 vial of 1 LDH with 1.1 ml or 3 ml of buffer, mix gently, and wait 5 minutes.
- Add 0.05 ml of sample to 1.0 ml of working reagent and mix.
- Read the absorbance at 340 nm at exactly 1 minute, then 30, 60 and 90 seconds after that.
- Work out the mean change in absorbance per minute (ΔA/min).
- LDH (IU/L) = ΔA/min × 3376.
Every stock code.
LDH ships in 2 pack sizes, each with its own stock code.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| CC1-LDK.014 | 20 x 1.1 ml | — | Datasheet |
| CC2-LDK.14U | 20 x 3 ml | — | Datasheet |
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