Name: Creatinine (Enzymatic)
Brand: Reckon Diagnostics
SKU: CC3-CEN.024

Reagent bottle: no photograph of this pack is published yet

Drawing, not a photograph. This pack has not been photographed yet; the stock codes and pack sizes below are the specification.

3 pack sizes, 40 to 240 ml.

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2 x 20 mlCC3-CEN.024 2 x 40 mlCC3-CEN.24U 3 x 80 mlCC3-CEN.24V

Stock code CC3-CEN.024

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Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

Creatinine (Enzymatic) is a reagent for the in vitro quantitative detection of creatinine in human serum on automated clinical chemistry analyzers, developed to avoid the chromogen interference of the Jaffe method. Creatininase, creatinase and sarcosine oxidase release hydrogen peroxide, which with peroxidase forms a quinoneimine dye read at 546 nm in proportion to creatinine. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Enzymatic colorimetric
Sample: Fresh serum without hemolysis
Sample volume: 3 µl
Wavelength: 546 nm (700 nm secondary)
Linearity: Up to 17 mg/dl
Storage: 2 to 8 °C

Full specification17 items

Category: Clinical chemistry
Pack: 40 to 240 ml
Pack sizes: 3 to choose from
Datasheet: Published
Method: Enzymatic colorimetric, end point
Sample: Fresh serum without hemolysis
Sample volume: 3 µl
Reagent volume: 150 µl of R1, then 50 µl of R2
Wavelength: 546 nm (700 nm secondary)
Temperature: 37 °C
Incubation: 5 minutes with R1, then 5 minutes after R2
Calibration: Two-point; liquid calibrator included
Reagents: R1 and R2, liquid, ready to use
On-board stability: 30 days
Storage: 2 to 8 °C; do not freeze
Stock codes: CC3-CEN.024, CC3-CEN.24U, CC3-CEN.24V
Made in: Vadodara, Gujarat, India

Performance3 figures

Linearity: Up to 17 mg/dl
Precision: CV ≤ 5%; inter-batch deviation R ≤ 10%
Interference: None up to ascorbic acid 0.5 g/L, bilirubin 0.4 g/L, hemoglobin 5 g/L, triglyceride 10 g/L

Procedure at a glance4 steps

Mix 3 µl of sample with 150 µl of R1 and incubate for 5 minutes at 37 °C; read absorbance A1.
Add 50 µl of R2, mix, and incubate for another 5 minutes; read absorbance A2.
Work out ΔA = A2 − A1 at 546 nm (700 nm secondary).
Creatinine (mg/dl) = ΔA of sample ÷ ΔA of calibrator × calibrator concentration.