Widal Slide Test (8 antigen, +ve & -ve control)

Photographed: the 8 x 5 ml pack, stock code IS-SAW.78T.

Supplied as 40 ml.

Pack size

8 x 5 mlIS-SAW.78T

Stock code IS-SAW.78T

Ask about this product → Datasheet (PDF) (opens in a new tab)

Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

Intended use: The Widal Slide Test (8 antigen, +ve and −ve control) is a slide and tube agglutination kit for detecting antibodies to Salmonella antigens in serum, with S. typhi O and H and S. paratyphi AO, BO, CO, AH, BH and CH suspensions. It screens on the slide and titrates on the slide or in tubes. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Slide and tube agglutination
Sample: Fresh serum
Sample volume: 1 drop of serum to screen
Reading time: 1 minute on the slide
Storage: 2 to 8 °C
Pack: 40 ml

Full specification14 items

Category: Serology
Pack: 40 ml
Datasheet: Published
Method: Slide and tube agglutination, Salmonella antigens
Antigens: S. typhi O and H; S. paratyphi AO, BO, CO, AH, BH and CH; 5 ml each
Sample: Fresh serum, not inactivated, hemolysed or turbid; up to 72 hours at 2 to 8 °C
Sample volume: 1 drop of serum to screen; 0.08 to 0.005 ml for a slide titre
Reading time: 1 minute on the slide; 16 to 20 hours at 37 °C in tubes
Titre range: 1:20 to 1:320 on the slide; 1:20 to 1:1280 in tubes
Controls: Positive and negative controls included, 1 ml each
Limitation: Confirm slide positives by the tube test; TAB vaccination, earlier infection or unrelated fevers can raise titres; agglutinins usually appear by the end of the first week; reading after 1 minute can give false positives
Storage: 2 to 8 °C, until the expiry date on each label
Stock codes: IS-SAW.78T
Made in: Vadodara, Gujarat, India

Performance1 figure

Reproducibility: 100% (± one double dilution)

Procedure at a glance5 steps

Bring all reagents to room temperature and shake each antigen vial well.
Place 1 drop of positive control in one circle, and 1 drop of serum and of saline in the circles needed.
Add 1 drop of the matching Widal antigen to each circle, mix with separate sticks, and rock the slide for 1 minute.
If the control and the serum agglutinate, titrate: 0.08, 0.04, 0.02, 0.01 and 0.005 ml of serum, each with 1 drop of antigen, give titres of 1:20 to 1:320.
In tubes, dilute the serum 1:20 to 1:1280 in saline, add 1 drop of antigen, incubate at 37 °C for 16 to 20 hours, and read the highest dilution with visible agglutination.

Every stock code.

Widal Slide Test (8 antigen, +ve & -ve control) ships in one pack size.

Stock codes and pack sizes for Widal Slide Test (8 antigen, +ve & -ve control)
Stock code	Pack	Photograph	Datasheet
IS-SAW.78T	8 x 5 ml		Datasheet PDF, IS-SAW.78T

Questions and answers.

Widal Slide Test (8 antigen, +ve & -ve control): what sample does it need?

The datasheet gives the sample as Fresh serum, not inactivated, hemolysed or turbid; up to 72 hours at 2 to 8 °C. The sample volume is 1 drop of serum to screen; 0.08 to 0.005 ml for a slide titre.

Widal Slide Test (8 antigen, +ve & -ve control): what method does it use?

The method on the datasheet is Slide and tube agglutination, Salmonella antigens.

Widal Slide Test (8 antigen, +ve & -ve control): how should it be stored?

The datasheet gives storage as 2 to 8 °C, until the expiry date on each label.

Widal Slide Test (8 antigen, +ve & -ve control): what pack sizes are available?

It comes in one pack size: 8 x 5 ml (IS-SAW.78T).