Photographed: the 50 Test pack, stock code RDT-HIC.103.
Card format. Supplied as 50 tests.
Stock code RDT-HIC.103
- Made and batch-released in Vadodara
- ISO 13485:2016 quality system
- Datasheet published: see every stock code below
Product details.
Intended use: The HIV 1/2 Triline Card is a solid-phase immunochromatographic assay for the qualitative detection of antibodies to HIV-1 and HIV-2 in human serum or plasma. Recombinant HIV-1 (gp120 + gp41) and HIV-2 (gp36) antigens sit on separate T1 and T2 lines, so the card tells HIV-1, HIV-2 and dual reactivity apart. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.
The specifications and steps on this page are from the datasheet, last updated 07-2025. The datasheet governs: read it before use, or ask us.
- Method
- Chromatographic lateral flow
- Sample
- Serum or plasma
- Sample volume
- 10 µl
- Read at
- 15 to 20 minutes
- Storage
- 2 to 40 °C
- Format
- Card
Full specification15 items
- Category
- Rapid diagnostics
- Format
- CARD
- Pack
- 50 tests
- Datasheet
- Published
- Method
- Chromatographic lateral flow, colloidal gold
- Detects
- Antibodies to HIV-1 (line T1) and HIV-2 (line T2)
- Sample
- Serum or plasma; refrigerate if not tested at once, and freeze beyond 3 days
- Sample volume
- 10 µl
- Buffer
- 2 drops (60 µl), 1 more if needed
- Read at
- 15 to 20 minutes; not after 20 minutes
- Limitation
- A screening test: repeat positives need confirmatory testing; very strong positives can show a hook effect (dilute 5 to 10 times)
- Storage
- 2 to 40 °C in the sealed pouch
- Caution
- Sensitive to humidity and heat: use as soon as the pouch is opened
- Stock codes
- RDT-HIC.103
- Made in
- Vadodara, Gujarat, India
Performance2 figures
- Agreement
- 99.8% against another commercial kit: 1 discordant result in 376 clinical samples
- Cross-reactivity
- None detected from other antigens, lipemic or icteric samples
Procedure at a glance5 steps
- Bring the card, sample and buffer to room temperature, and lay the card flat.
- Add 10 µl of serum or plasma to the sample well, with the dropper or a micropipette.
- Add 2 drops (60 µl) of assay buffer to the sample well; add 1 more drop if needed.
- Read at 15 to 20 minutes, not later.
- C only is negative; C and T1 is HIV-1 positive; C and T2 is HIV-2 positive; C, T1 and T2 is positive for both. No C line means the test is invalid.
Every stock code.
HIV 1/2 Triline Card ships in one pack size.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| RDT-HIC.103 | 50 Test |  |
Datasheet PDF, RDT-HIC.103 |
Questions and answers.
HIV 1/2 Triline Card: what sample does it need?
The datasheet gives the sample as Serum or plasma; refrigerate if not tested at once, and freeze beyond 3 days. The sample volume is 10 µl.
HIV 1/2 Triline Card: what method does it use?
The method on the datasheet is Chromatographic lateral flow, colloidal gold.
HIV 1/2 Triline Card: how should it be stored?
The datasheet gives storage as 2 to 40 °C in the sealed pouch.
HIV 1/2 Triline Card: what pack sizes are available?
It comes in one pack size: 50 Test (RDT-HIC.103).
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