Name: HBsAg Whole Blood (Hepa Card)
Brand: Reckon Diagnostics
SKU: RDT-HEC.73W

Photographed: the 50 Test pack, stock code RDT-HEC.73W.

CARD format. supplied as 50 tests.

Pack size

50 TestRDT-HEC.73W

Stock code RDT-HEC.73W

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Made and batch-released in Vadodara
ISO 13485:2016 quality system
Datasheet published: see every stock code below

Product details.

The HBsAg (WB) (Hepa Card) is a one-step immunochromatographic sandwich assay for the qualitative detection of hepatitis B surface antigen (HBsAg) in whole blood, serum or plasma. A monoclonal antibody gold conjugate and polyclonal solid-phase antibodies bind HBsAg to form a pink-purple test line, with a goat anti-mouse IgG control line. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 01-2026. The datasheet governs: read it before use, or ask us.

Method: Antigen capture sandwich
Detects: Hepatitis B surface antigen (HBsAg)
Sample: Whole blood
Sample volume: 1 drop (about 25 µl) of whole blood
Read at: Within 20 minutes
Sensitivity: 0.5 ng/ml HBsAg

Full specification15 items

Category: Rapid diagnostics
Format: CARD
Pack: 50 tests
Datasheet: Published
Method: Antigen capture sandwich, colloidal gold
Detects: Hepatitis B surface antigen (HBsAg)
Sample: Whole blood (finger prick, or EDTA, heparin or oxalate tube), serum or plasma; whole blood keeps 72 hours at 2 to 8 °C; not hemolysed or clotted
Sample volume: 1 drop (about 25 µl) of whole blood; 2 to 3 drops of serum or plasma
Buffer: 2 to 3 drops (60 to 90 µl) for whole blood, 1 more if needed
Read at: Within 20 minutes; strong positives may show within 5 minutes
Limitation: Shows only the presence or absence of HBsAg; if negative with persisting symptoms, repeat on a fresh sample
Storage: 2 to 40 °C in the sealed pouch
Caution: Handle specimens as infectious; soak used material in 0.5 to 1% sodium hypochlorite for 1 hour
Stock codes: RDT-HEC.73W
Made in: Vadodara, Gujarat, India

Performance1 figure

Sensitivity: 0.5 ng/ml HBsAg

Procedure at a glance5 steps

Bring the card and the sample to room temperature, and lay the card flat.
Whole blood: add 1 drop (about 25 µl), wait about 30 seconds for it to soak in, then add 2 to 3 drops (60 to 90 µl) of buffer.
Serum or plasma: add 2 to 3 drops of sample to the sample well, without overflowing.
Read within 20 minutes; strong positives may show within 5 minutes.
C only is negative; C and T is positive for HBsAg; no C line means the test is invalid.