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ASO Turbilatex

ASO Turbilatex, 1 x 50 ml ASO Turbilatex, 1 x 50 ml, view 2

Photographed: the 1 x 50 ml pack, stock code IM-AST.081.

Supplied as 50 ml.

Pack size

Stock code IM-AST.081

  • Made and batch-released in Vadodara
  • ISO 13485:2016 quality system
  • Datasheet published: see every stock code below

Product details.

Intended use: ASO Turbilatex is a quantitative latex turbidimetric test for anti-streptolysin O (ASO) in human serum or plasma. Latex particles coated with streptolysin O agglutinate with the ASO in the sample, and the change in absorbance is compared with a calibrator of known ASO concentration. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method
Latex turbidimetry
Sample
Fresh serum
Sample volume
10 µl
Wavelength
540 nm (530 to 550 nm)
Linearity
Up to 800 IU/ml
Sensitivity
Δ 0.73 mA per IU/ml
Full specification16 items
Pack
50 ml
Datasheet
Published
Method
Latex turbidimetry, two point (fixed time)
Sample
Fresh serum: stable 7 days at 2 to 8 °C or 3 months at −20 °C; centrifuge samples with fibrin; not highly hemolysed or lipemic
Sample volume
10 µl
Reagent volume
1 ml of working reagent (0.9 ml of R1 + 0.1 ml of R2)
Wavelength
540 nm (530 to 550 nm)
Temperature
37 °C
Readings
Immediately, and again after 2 minutes
Calibration
Calibrator included, 310 IU/ml (value may vary by lot)
Working reagent
30 days at 2 to 8 °C
Caution
Human-origin components tested negative for HBsAg, HCV and HIV; handle as potentially infectious
Storage
2 to 8 °C; do not freeze
Stock codes
IM-AST.081
Made in
Vadodara, Gujarat, India
Performance4 figures
Linearity
Up to 800 IU/ml; dilute higher samples 1/3 in 9 g/L NaCl
Detection limit
20 IU/ml
Sensitivity
Δ 0.73 mA per IU/ml
Interference
None from bilirubin below 20 mg/dl, hemoglobin below 10 g/L, lipemia below 10 g/L, rheumatoid factors up to 600 IU/ml
Procedure at a glance4 steps
  1. Mix 0.9 ml of diluent (R1) with 0.1 ml of latex (R2) as the working reagent; the calibrator is ready to use.
  2. Add 10 µl of calibrator or sample to 1.0 ml of working reagent and mix.
  3. Read the absorbance at 540 nm immediately (A1) and again after 2 minutes (A2).
  4. ASO (IU/ml) = (A2 − A1) of sample ÷ (A2 − A1) of calibrator × calibrator concentration.

Every stock code.

ASO Turbilatex ships in one pack size.

Stock codes and pack sizes for ASO Turbilatex
Stock code Pack Photograph Datasheet
IM-AST.081 1 x 50 ml ASO Turbilatex, 1 x 50 ml Datasheet PDF, IM-AST.081

Questions and answers.

ASO Turbilatex: what sample does it need?

The datasheet gives the sample as Fresh serum: stable 7 days at 2 to 8 °C or 3 months at −20 °C; centrifuge samples with fibrin; not highly hemolysed or lipemic. The sample volume is 10 µl.

ASO Turbilatex: what method does it use?

The method on the datasheet is Latex turbidimetry, two point (fixed time).

ASO Turbilatex: what wavelength is it read at?

According to the datasheet, it is read at 540 nm (530 to 550 nm).

ASO Turbilatex: what is the linear range?

The datasheet gives the linear range as Up to 800 IU/ml; dilute higher samples 1/3 in 9 g/L NaCl.

ASO Turbilatex: how should it be stored?

The datasheet gives storage as 2 to 8 °C; do not freeze.

ASO Turbilatex: what pack sizes are available?

It comes in one pack size: 1 x 50 ml (IM-AST.081).

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