Product details.

ASO Turbilatex is a quantitative latex turbidimetric test for anti-streptolysin O (ASO) in human serum or plasma. Latex particles coated with streptolysin O agglutinate with the ASO in the sample, and the change in absorbance is compared with a calibrator of known ASO concentration. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 04-2023. The datasheet governs: read it before use, or ask us.

Method: Latex turbidimetry
Sample: Fresh serum
Sample volume: 10 µl
Wavelength: 540 nm (530 to 550 nm)
Linearity: Up to 800 IU/ml
Sensitivity: Δ 0.73 mA per IU/ml

Full specification16 items

Category: Immunoturbidimetric
Pack: 50 ml
Datasheet: Published
Method: Latex turbidimetry, two point (fixed time)
Sample: Fresh serum: stable 7 days at 2 to 8 °C or 3 months at −20 °C; centrifuge samples with fibrin; not highly hemolysed or lipemic
Sample volume: 10 µl
Reagent volume: 1 ml of working reagent (0.9 ml of R1 + 0.1 ml of R2)
Wavelength: 540 nm (530 to 550 nm)
Temperature: 37 °C
Readings: Immediately, and again after 2 minutes
Calibration: Calibrator included, 310 IU/ml (value may vary by lot)
Working reagent: 30 days at 2 to 8 °C
Caution: Human-origin components tested negative for HBsAg, HCV and HIV; handle as potentially infectious
Storage: 2 to 8 °C; do not freeze
Stock codes: IM-AST.081
Made in: Vadodara, Gujarat, India

Performance4 figures

Linearity: Up to 800 IU/ml; dilute higher samples 1/3 in 9 g/L NaCl
Detection limit: 20 IU/ml
Sensitivity: Δ 0.73 mA per IU/ml
Interference: None from bilirubin below 20 mg/dl, hemoglobin below 10 g/L, lipemia below 10 g/L, rheumatoid factors up to 600 IU/ml

Procedure at a glance4 steps

Mix 0.9 ml of diluent (R1) with 0.1 ml of latex (R2) as the working reagent; the calibrator is ready to use.
Add 10 µl of calibrator or sample to 1.0 ml of working reagent and mix.
Read the absorbance at 540 nm immediately (A1) and again after 2 minutes (A2).
ASO (IU/ml) = (A2 − A1) of sample ÷ (A2 − A1) of calibrator × calibrator concentration.