Photographed: the 1 x 50 ml pack, stock code IM-RFT.083.
Supplied as 50 ml.
Stock code IM-RFT.083
- Supplied by Reckon Diagnostics, Vadodara
- Datasheet published: see every stock code below
Product details.
Intended use: RF Turbilatex is a quantitative latex turbidimetric test for rheumatoid factors (RF) in human serum or plasma. Latex particles coated with human gammaglobulin agglutinate with the RF in the sample, and the change in absorbance is read against a multi-point or single-point calibration. Supplied by Reckon Diagnostics, Vadodara.
The specifications and steps on this page are from the datasheet, last updated 06-2023. The datasheet governs: read it before use, or ask us.
- Method
- Latex turbidimetry
- Sample
- Fresh serum
- Sample volume
- 7 µl
- Wavelength
- 650 nm (600 to 650 nm)
- Linearity
- Up to 100 IU/ml single point
- Measuring range
- 20 to 150 IU/ml on the calibration curve
Full specification17 items
- Category
- Immunoturbidimetric
- Pack
- 50 ml
- Datasheet
- Published
- Method
- Latex turbidimetry, end point (multi-standard)
- Sample
- Fresh serum: stable 7 days at 2 to 8 °C or 3 months at −20 °C; centrifuge samples with fibrin; not highly hemolysed or lipemic
- Sample volume
- 7 µl
- Reagent volume
- 1 ml of working reagent (0.9 ml of R1 + 0.1 ml of R2)
- Wavelength
- 650 nm (600 to 650 nm)
- Temperature
- 37 °C
- Incubation
- 2 minutes
- Calibration
- 6-point curve (20 to 150 IU/ml), or single point with the calibrator diluted to 45 IU/ml
- Working reagent
- 30 days at 2 to 8 °C
- Calibrator stability
- 1 month at 2 to 8 °C; 3 months at −20 °C
- Caution
- Human-origin components tested negative for HBsAg, HCV and HIV; handle as potentially infectious
- Storage
- 2 to 8 °C; do not freeze
- Stock codes
- IM-RFT.083
- Supplied by
- Reckon Diagnostics, Vadodara
Performance5 figures
- Linearity
- Up to 100 IU/ml single point; up to 150 IU/ml multi-point
- Measuring range
- 20 to 150 IU/ml on the calibration curve; dilute higher samples 1/5 in 9 g/L NaCl
- Detection limit
- 3 IU/ml
- Sensitivity
- Δ 3.34 mA per IU/ml
- Interference
- None from bilirubin 20 mg/dl, hemoglobin 10 g/L, lipemia 10 g/L
Procedure at a glance5 steps
- Mix 0.9 ml of diluent (R1) with 0.1 ml of latex (R2) as the working reagent.
- Prepare the 6 calibrator dilutions in 9 g/L NaCl for a multi-point curve, or mix 30 µl of calibrator with 70 µl of NaCl for a single point (45 IU/ml).
- Add 7 µl of calibrator or sample to 1.0 ml of working reagent (blank: 7 µl of NaCl) and mix.
- Read the blank (A1) and the sample or calibrator (A2) at 650 nm after 2 minutes.
- Read RF off the calibration curve of (A2 − A1), or RF (IU/ml) = (A2 − A1) of sample ÷ (A2 − A1) of calibrator × diluted calibrator concentration.
Every stock code.
RF Turbilatex ships in one pack size.
| Stock code | Pack | Photograph | Datasheet |
|---|---|---|---|
| IM-RFT.083 | 1 x 50 ml |  |
Datasheet PDF, IM-RFT.083 |
Questions and answers.
RF Turbilatex: what sample does it need?
The datasheet gives the sample as Fresh serum: stable 7 days at 2 to 8 °C or 3 months at −20 °C; centrifuge samples with fibrin; not highly hemolysed or lipemic. The sample volume is 7 µl.
RF Turbilatex: what method does it use?
The method on the datasheet is Latex turbidimetry, end point (multi-standard).
RF Turbilatex: what wavelength is it read at?
According to the datasheet, it is read at 650 nm (600 to 650 nm).
RF Turbilatex: what is the linear range?
The datasheet gives the linear range as Up to 100 IU/ml single point; up to 150 IU/ml multi-point.
RF Turbilatex: how should it be stored?
The datasheet gives storage as 2 to 8 °C; do not freeze.
RF Turbilatex: what pack sizes are available?
It comes in one pack size: 1 x 50 ml (IM-RFT.083).
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