Product details.

RF Turbilatex is a quantitative latex turbidimetric test for rheumatoid factors (RF) in human serum or plasma. Latex particles coated with human gammaglobulin agglutinate with the RF in the sample, and the change in absorbance is read against a multi-point or single-point calibration. Manufactured at Reckon’s Vadodara facility under ISO 13485:2016 quality management, and released against reference standards before dispatch.

The specifications and steps on this page are from the datasheet, last updated 06-2023. The datasheet governs: read it before use, or ask us.

Method: Latex turbidimetry
Sample: Fresh serum
Sample volume: 7 µl
Wavelength: 650 nm (600 to 650 nm)
Linearity: Up to 100 IU/ml single point
Measuring range: 20 to 150 IU/ml on the calibration curve

Full specification17 items

Category: Immunoturbidimetric
Pack: 50 ml
Datasheet: Published
Method: Latex turbidimetry, end point (multi-standard)
Sample: Fresh serum: stable 7 days at 2 to 8 °C or 3 months at −20 °C; centrifuge samples with fibrin; not highly hemolysed or lipemic
Sample volume: 7 µl
Reagent volume: 1 ml of working reagent (0.9 ml of R1 + 0.1 ml of R2)
Wavelength: 650 nm (600 to 650 nm)
Temperature: 37 °C
Incubation: 2 minutes
Calibration: 6-point curve (20 to 150 IU/ml), or single point with the calibrator diluted to 45 IU/ml
Working reagent: 30 days at 2 to 8 °C
Calibrator stability: 1 month at 2 to 8 °C; 3 months at −20 °C
Caution: Human-origin components tested negative for HBsAg, HCV and HIV; handle as potentially infectious
Storage: 2 to 8 °C; do not freeze
Stock codes: IM-RFT.083
Made in: Vadodara, Gujarat, India

Performance5 figures

Linearity: Up to 100 IU/ml single point; up to 150 IU/ml multi-point
Measuring range: 20 to 150 IU/ml on the calibration curve; dilute higher samples 1/5 in 9 g/L NaCl
Detection limit: 3 IU/ml
Sensitivity: Δ 3.34 mA per IU/ml
Interference: None from bilirubin 20 mg/dl, hemoglobin 10 g/L, lipemia 10 g/L

Procedure at a glance5 steps

Mix 0.9 ml of diluent (R1) with 0.1 ml of latex (R2) as the working reagent.
Prepare the 6 calibrator dilutions in 9 g/L NaCl for a multi-point curve, or mix 30 µl of calibrator with 70 µl of NaCl for a single point (45 IU/ml).
Add 7 µl of calibrator or sample to 1.0 ml of working reagent (blank: 7 µl of NaCl) and mix.
Read the blank (A1) and the sample or calibrator (A2) at 650 nm after 2 minutes.
Read RF off the calibration curve of (A2 − A1), or RF (IU/ml) = (A2 − A1) of sample ÷ (A2 − A1) of calibrator × diluted calibrator concentration.